Department of Food Science and Biotechnology, National Chung Hsing University, Taichung, Taiwan, ROC.
J Agric Food Chem. 2009 Oct 14;57(19):8852-9. doi: 10.1021/jf9028333.
Acteoside, an active phenylethanoid glycoside of many medicinal plants and bitter tea, displays anti-inflammatory properties in vitro. However, it is unclear whether acteoside and similar compounds may inhibit the expression of cell adhesion molecules (CAMs), which plays a role in the pathogenesis of atherosclerosis and inflammation. Here, we found that acteoside, isoacteoside, and 6-O-acetylacteoside inhibited IL-1beta-activated expression of intercellular CAM-1 (ICAM-1) and vascular CAM-1 (VCAM-1) in human umbilical vein endothelial cells (HUVECs); the inhibitory potency was as follows: 6-O-acetylacteoside > acteoside > isoacteoside. Acteoside and 6-O-acetylacteoside also dose-dependently inhibited VCAM-1 gene promoter activity in IL-1beta-activated HUVECs. The inhibition of acteoside and 6-O-acetylacteoside on IL-1beta-activated expression of CAMs was manifested by decreased phosphorylation of extracellular signal-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK). These results indicate that acteoside and 6-O-acetylacteoside may exert anti-inflammatory activities in vascular endothelium by inhibiting the expression of CAMs, primarily through decreased phosphorylation of ERK and JNK.
毛蕊花糖苷是许多药用植物和苦茶中的一种活性苯乙醇苷，具有体外抗炎特性。然而，尚不清楚毛蕊花糖苷和类似化合物是否可以抑制细胞黏附分子（CAM）的表达，CAM 在动脉粥样硬化和炎症的发病机制中起作用。在这里，我们发现毛蕊花糖苷、异毛蕊花糖苷和 6-O-乙酰毛蕊花糖苷抑制了人脐静脉内皮细胞（HUVEC）中白细胞介素-1β（IL-1β）激活的细胞间黏附分子-1（ICAM-1）和血管细胞黏附分子-1（VCAM-1）的表达；抑制效力如下：6-O-乙酰毛蕊花糖苷>毛蕊花糖苷>异毛蕊花糖苷。毛蕊花糖苷和 6-O-乙酰毛蕊花糖苷还剂量依赖性地抑制了 IL-1β激活的 HUVEC 中 VCAM-1 基因启动子活性。毛蕊花糖苷和 6-O-乙酰毛蕊花糖苷对 IL-1β激活的 CAM 表达的抑制作用表现为细胞外信号调节激酶（ERK）和 c-Jun N-末端激酶（JNK）的磷酸化减少。这些结果表明，毛蕊花糖苷和 6-O-乙酰毛蕊花糖苷可能通过抑制 CAM 的表达在血管内皮中发挥抗炎活性，主要通过 ERK 和 JNK 的磷酸化减少。
