Bertschinger H U, Bachmann M, Mettler C, Pospischil A, Schraner E M, Stamm M, Sydler T, Wild P
Institute of Veterinary Bacteriology, University of Zürich, Switzerland.
Vet Microbiol. 1990 Nov;25(2-3):267-81. doi: 10.1016/0378-1135(90)90083-8.
Two strains of E. coli O139:K12 (B):H1 were compared in vitro and in the intestinal environment. Both strains colonized the small intestines of experimentally inoculated pigs and exhibited in vivo a similar relationship to the microvillus border as enterotoxigenic E. coli (ETEC). Strain 107/86 grown on blood agar expressed numerous long flexible non-haemagglutinating fimbriae which were antigenically distinct from the known fimbriae of porcine ETEC. It adhered in vitro to porcine enterocyte brush border fragments. Strain 124/76 grown on blood agar was devoid of fimbriae and did not adhere to brush border fragments. However, fimbriae morphologically and antigenically indistinguishable from those of strain 107/86 were detected in the intestinal environment by direct immunofluorescence and by immuno electron microscopy.
对两株大肠杆菌O139:K12（B）:H1进行了体外和肠道环境中的比较。两株菌均在实验接种猪的小肠中定殖，并且在体内与产肠毒素大肠杆菌（ETEC）一样，与微绒毛边界呈现相似的关系。在血琼脂上生长的107/86菌株表达大量长而柔韧的非血凝性菌毛，这些菌毛在抗原性上与猪ETEC已知的菌毛不同。它在体外能黏附于猪肠上皮细胞刷状缘片段。在血琼脂上生长的124/76菌株没有菌毛，也不黏附于刷状缘片段。然而，通过直接免疫荧光和免疫电子显微镜在肠道环境中检测到了形态和抗原性与107/86菌株无法区分的菌毛。
