NIH 化学基因组学中心的试点阶段。
The pilot phase of the NIH Chemical Genomics Center.
机构信息
NIH Chemical Genomics Center, NHGRI, National Institutes of Health, 9800 Medical Center Drive, Building B, Room 3005, MSC: 3370, Bethesda, MD 20892-3370, USA.
出版信息
Curr Top Med Chem. 2009;9(13):1181-93. doi: 10.2174/156802609789753644.
Abstract
The NIH Chemical Genomics Center (NCGC) was the inaugural center of the Molecular Libraries and Screening Center Network (MLSCN). Along with the nine other research centers of the MLSCN, the NCGC was established with a primary goal of bringing industrial technology and experience to empower the scientific community with small molecule compounds for use in their research. We intend this review to serve as 1) an introduction to the NCGC standard operating procedures, 2) an overview of several of the lessons learned during the pilot phase and 3) a review of several of the innovative discoveries reported during the pilot phase of the MLSCN.
摘要
美国国立卫生研究院化学基因组学中心（NCGC）是分子库和筛选中心网络（MLSCN）的创始中心之一。与 MLSCN 的其他九个研究中心一起，NCGC 的成立主要目标是为科学界提供工业技术和经验，以小分子化合物为他们的研究提供支持。我们希望本综述能够：1）介绍 NCGC 的标准操作程序，2）概述在试点阶段学到的一些经验教训，3）回顾 MLSCN 试点阶段报告的一些创新发现。
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2
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