de Sanctis Juan B, Garmendia Jenny V, Chaurio Ricardo, Zabaleta Mercedes, Rivas Liliana
Facultad de Medicina, Instituto de Inmunología, University of Central de Venezuela, Caracas, Venezuela.
Autoimmunity. 2009 May;42(4):263-5. doi: 10.1080/08916930902827942.
CD154, a member of the tumor necrosis factor receptor family, is involved in several biological responses. In the sera of systemic lupus erythematosus (SLE) patients, the levels of sCD154 have been shown to be increased, however, few reports have dealt with the biologically active tetramer. Here, we assessed the biological activity of the serum CD154 tetramer using bioassays for BC activation and production nitrite or peroxide. The patients showed a markedly increased total sCD154 serum concentration (12.5 +/- 8.2 vs. 3.9 +/- 1.2 ng/ml; p < 0.001). ba-sCD154 was significantly increased in non-treated patients (7.4 +/- 3.4 ng/ml, n = 22; p < 0.001) and patients with the highest SLE disease activity index (SLEDAI) scores (5.3 +/- 2.9 ng/ml, n = 8), but not in stable patients (1.3 +/- 1.2 ng/ml, n = 30) whose values were similar to normal healthy donors (NHD; 0.8 +/- 0.2 ng/ml). Patients with SLEDAI above 8 that recovered after successful treatment displayed significantly decreased levels of ba-sCD154. We conclude that the bioassay is a useful tool discriminating active and stable SLE, as well as non-treated patients.
CD154是肿瘤坏死因子受体家族的一员，参与多种生物学反应。在系统性红斑狼疮（SLE）患者的血清中，可溶性CD154（sCD154）水平已被证明有所升高，然而，关于具有生物活性的四聚体的报道却很少。在此，我们使用BC激活和亚硝酸盐或过氧化物生成的生物测定法评估了血清CD154四聚体的生物活性。患者的血清总sCD154浓度显著升高（12.5±8.2 vs. 3.9±1.2 ng/ml；p<0.001）。未治疗患者（7.4±3.4 ng/ml，n = 22；p<0.001）和SLE疾病活动指数（SLEDAI）得分最高的患者（5.3±2.9 ng/ml，n = 8）的生物活性sCD154（ba-sCD154）显著升高，但病情稳定的患者（1.3±1.2 ng/ml，n = 30）中未升高，其值与正常健康供体（NHD；0.8±0.2 ng/ml）相似。成功治疗后康复且SLEDAI高于8的患者，其ba-sCD154水平显著降低。我们得出结论，该生物测定法是区分活动期和稳定期SLE以及未治疗患者的有用工具。
