Lemay P, Pluquet de Temmerman N, Tavitian A, Bernard J
C R Acad Hebd Seances Acad Sci D. 1977 Jun 27;284(24):2573-5.
A DNA complementary to the viral genome of C-type particles produced by a Mouse myeloma derived cell line (MF2 cell line) was synthesized. This cDNA was used as a probe to study the viral genome expression among the total RNA and the poly (A)-rich RNA extracted from the MF2 and Balb/c embryonic cells. As evidenced by molecular hybridization experiments, the presence of at least one endogenous Balb/c virus in the MF2 virus stocks is suggested. In the productive cells, the viral RNA sequences are expressed in the poly (A)-rich RNA fraction.
合成了与小鼠骨髓瘤衍生细胞系(MF2细胞系)产生的C型颗粒病毒基因组互补的DNA。该cDNA用作探针,研究从MF2和Balb/c胚胎细胞中提取的总RNA和富含多聚腺苷酸(poly (A))的RNA中的病毒基因组表达。分子杂交实验表明,MF2病毒株中至少存在一种内源性Balb/c病毒。在产生病毒的细胞中,病毒RNA序列在富含多聚腺苷酸的RNA组分中表达。