Ramabhadran T V, Hartley J W, Rowe W P, Godefroy-Colburn T, Jhabvala P S, Thach R E
J Virol. 1979 Oct;32(1):123-30. doi: 10.1128/JVI.32.1.123-130.1979.
MOPC-460 mouse plasmacytoma cells produce intracellular A-type particles and extracellular oncornavirus-like particles ("myeloma-associated virus," abbreviated MAV). The genomes of these two particles are closely related. During attempts to establish infections with MOPC-460 extracellular particles, we isolated ecotropic and xenotropic infectious forms of murine leukemia virus. We have investigated the relation of these isolates to A-type particles and to MAV by nucleic acid hybridization. Using complementary DNA probes prepared from the two isolates, we found that these infectious murine leukemia viruses differ from A-type particles and from MAV. Moreover, we found that MAV is the predominant extracellular component: the ecotropic and xenotropic forms of murine leukemia virus were present at only low levels (less than 5%) in MAV preparations. Neither the SC-1 cells infected with ectropic murine leukemia virus nor the mink cells infected with xenotropic murine leukemia virus showed any A-type particles in their cytoplasm when examined by electron microscopy. Our inability to demonstrate infection by the A-type particle-related component, MAV, suggests that these may be defective.
MOPC - 460小鼠浆细胞瘤细胞产生细胞内A型颗粒和细胞外类肿瘤病毒颗粒（“骨髓瘤相关病毒”，缩写为MAV）。这两种颗粒的基因组密切相关。在尝试用MOPC - 460细胞外颗粒建立感染的过程中，我们分离出了亲嗜性和异嗜性感染形式的鼠白血病病毒。我们通过核酸杂交研究了这些分离株与A型颗粒以及MAV之间的关系。使用从这两种分离株制备的互补DNA探针，我们发现这些感染性鼠白血病病毒不同于A型颗粒和MAV。此外，我们发现MAV是主要的细胞外成分：在MAV制剂中，亲嗜性和异嗜性形式的鼠白血病病毒仅以低水平（低于5%）存在。当通过电子显微镜检查时，感染亲嗜性鼠白血病病毒的SC - 1细胞和感染异嗜性鼠白血病病毒的貂细胞在其细胞质中均未显示出任何A型颗粒。我们无法证明与A型颗粒相关的成分MAV具有感染性，这表明它们可能存在缺陷。
