Miljkovic Natasa D, Cooper Gregory M, Hott Shelby L, Disalle Brian F, Gawalt Ellen S, Smith Darren M, McGowan Kenneth, Marra Kacey G
Division of Plastic Surgery, Department of Surgery, University of Pittsburgh, 200 Lothrop St., Pittsburgh, PA 15261, USA.
J Craniofac Surg. 2009 Sep;20(5):1538-43. doi: 10.1097/SCS.0b013e3181b09c13.
Calcium aluminate (CaAl), arginine-glycine-aspartic acid-modified CaAl, and beta-tricalcium phosphate (TCP) implants were studied in a rat calvarial critical-sized defect model. The rates of newly formed bone and osteointegration were measured using 3 different methods: radiography, micro-computed tomography, and histologic examination. After 4 weeks, there was no new bone formed and no signs of osteointegration into the skull bone in the CaAl or arginine-glycine-aspartic acid-modified CaAl groups, and thick fibrous capsules were visible around the whole circumference of the implants in both groups. In the beta-TCP group, neovascularization of the implant was observed, which is consistent with the early phase of new bone formation. In addition, in the beta-TCP group, signs of implant integration into the host tissue were evident at 4 weeks. There was no soft tissue reaction around the beta-TCP implant. These observations suggest that more specific adhesion peptides may be needed to activate the bioinert CaAl implant and promote bone formation in the craniofacial skeleton.
在大鼠颅骨临界尺寸缺损模型中研究了铝酸钙（CaAl）、精氨酸 - 甘氨酸 - 天冬氨酸修饰的CaAl以及β - 磷酸三钙（TCP）植入物。使用三种不同方法测量新形成骨的速率和骨整合情况：放射照相术、微型计算机断层扫描和组织学检查。4周后，CaAl组或精氨酸 - 甘氨酸 - 天冬氨酸修饰的CaAl组均未形成新骨，也没有骨整合到颅骨中的迹象，两组植入物的整个圆周周围都可见厚厚的纤维囊。在β - TCP组中，观察到植入物有新血管形成，这与新骨形成的早期阶段一致。此外，在β - TCP组中，4周时植入物与宿主组织整合的迹象明显。β - TCP植入物周围没有软组织反应。这些观察结果表明，可能需要更特异的粘附肽来激活生物惰性的CaAl植入物并促进颅面骨骼中的骨形成。
