Park Jin-Woo, Kim Jae-Min, Lee Heon-Jin, Jeong Seong-Hwa, Suh Jo-Young, Hanawa Takao
Department of Periodontology, School of Dentistry, Kyungpook National University, Daegu, Korea.
J Clin Periodontol. 2014 Feb;41(2):181-90. doi: 10.1111/jcpe.12198. Epub 2013 Dec 11.
This study investigated the efficacy of the hypothalamic nonapeptide oxytocin (OT) by direct delivery to local defects using a microporous β-tricalcium phosphate (TCP) as the carrier for the future applications as a method to achieve predictable bone regeneration of large osseous defects requiring sinus bone graft and guided bone regeneration procedures for implant placement.
Both the ectopic and new bone formation induced by the OT-loaded microporous β-TCP powder was histomorphometrically compared with unloaded β-TCP in a subcutaneous ectopic bone formation model and calvarial critical-sized defects (CSDs) in 45 rats.
The OT-loaded β-TCP clearly enhanced ectopic bone formation compared with the unloaded control group. A High initial OT dose (250 μg) significantly increased ectopic bone formation at an early healing time-point compared with a lower OT dose (50 μg). The OT-loaded samples displayed greater new bone formation in the rat calvarial CSDs. Extensive new bone formation was achieved in the calvarial CSDs with the higher OT dose.
These results suggest that local OT delivery to bone substitute promotes new bone formation via an osteoinductive mode of action.
本研究通过使用微孔β - 磷酸三钙（TCP）作为载体将下丘脑九肽催产素（OT）直接递送至局部缺损部位，来研究其疗效，以期未来作为一种方法用于实现需要鼻窦骨移植和引导骨再生程序以进行种植体植入的大型骨缺损的可预测性骨再生。
在45只大鼠的皮下异位骨形成模型和颅骨临界尺寸缺损（CSD）中，对负载OT的微孔β - TCP粉末诱导的异位和新骨形成与未负载的β - TCP进行组织形态计量学比较。
与未负载的对照组相比，负载OT的β - TCP明显增强了异位骨形成。与较低的OT剂量（50μg）相比，高初始OT剂量（250μg）在早期愈合时间点显著增加了异位骨形成。负载OT的样本在大鼠颅骨CSD中显示出更大的新骨形成。较高OT剂量的颅骨CSD实现了广泛的新骨形成。
这些结果表明，将OT局部递送至骨替代物可通过骨诱导作用方式促进新骨形成。
