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寡核苷酸探针在DRB亚区HLA - II类分型中的应用。

The application of oligonucleotide probes to HLA class II typing of the DRB sub-region.

作者信息

Vaughan R W, Lanchbury J S, Marsh S G, Hall M A, Bodmer J G, Welsh K I

机构信息

Dept. Molecular Immunogenetics, Guy's Hospital, London, U.K.

出版信息

Tissue Antigens. 1990 Oct;36(4):149-55. doi: 10.1111/j.1399-0039.1990.tb01821.x.

DOI:10.1111/j.1399-0039.1990.tb01821.x
PMID:1981815
Abstract

A plan for DRB typing at the sequence level is detailed. Only one polymerase chain amplification reaction is needed and the application of a limited number of short oligonucleotide probes allows an almost complete definition of DRB alleles. The scheme was tested on 40 homozygous cell-lines selected to cover a wide range of specificities, and 40 RFLP-typed controls. The results are presented and discussed. The simplicity and accuracy of this scheme are emphasized.

摘要

详细介绍了在序列水平上进行DRB分型的方案。仅需一次聚合酶链扩增反应，使用有限数量的短寡核苷酸探针就能几乎完全确定DRB等位基因。该方案在40个为涵盖广泛特异性而选择的纯合细胞系以及40个经RFLP分型的对照上进行了测试。展示并讨论了结果。强调了该方案的简单性和准确性。

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