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苏云金芽孢杆菌 Cry11Aa 寡聚化在其对埃及伊蚊的毒性中起重要作用。

Oligomerization of Cry11Aa from Bacillus thuringiensis has an important role in toxicity against Aedes aegypti.

机构信息

Universidad Nacional Autónoma de México, Cuernavaca 62250, Morelos, Mexico.

出版信息

Appl Environ Microbiol. 2009 Dec;75(23):7548-50. doi: 10.1128/AEM.01303-09. Epub 2009 Oct 9.

DOI:10.1128/AEM.01303-09
PMID:19820153
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2786423/
Abstract

Cry11Aa and Cyt1Aa of Bacillus thuringiensis are active against mosquitoes and show synergism. Cyt1Aa functions as a membrane receptor inducing Cry11Aa oligomerization. Here we characterized Cry11Aa helix alpha-3 mutants impaired in oligomerization and toxicity against Aedes aegypti, indicating that oligomerization of Cry11Aa is important for toxin action. Cyt1Aa did not recover the insecticidal activity of Cry11Aa mutants.

摘要

苏云金芽孢杆菌的 Cry11Aa 和 Cyt1Aa 对蚊子具有活性，并表现出协同作用。Cyt1Aa 作为一种膜受体，诱导 Cry11Aa 寡聚化。在这里，我们对 Cry11Aa 螺旋 α-3 突变体进行了表征，这些突变体在寡聚化和对埃及伊蚊的毒性方面存在缺陷，这表明 Cry11Aa 的寡聚化对于毒素作用很重要。Cyt1Aa 不能恢复 Cry11Aa 突变体的杀虫活性。

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Oligomerization of Cry11Aa from Bacillus thuringiensis has an important role in toxicity against Aedes aegypti.苏云金芽孢杆菌 Cry11Aa 寡聚化在其对埃及伊蚊的毒性中起重要作用。
Appl Environ Microbiol. 2009 Dec;75(23):7548-50. doi: 10.1128/AEM.01303-09. Epub 2009 Oct 9.
2
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本文引用的文献

1
Bacillus thuringiensis ssp. israelensis Cyt1Aa enhances activity of Cry11Aa toxin by facilitating the formation of a pre-pore oligomeric structure.苏云金芽孢杆菌以色列亚种Cyt1Aa通过促进孔前寡聚体结构的形成来增强Cry11Aa毒素的活性。
Cell Microbiol. 2007 Dec;9(12):2931-7. doi: 10.1111/j.1462-5822.2007.01007.x. Epub 2007 Aug 2.
2
Bacillus thuringiensis Cry1Ab mutants affecting oligomer formation are non-toxic to Manduca sexta larvae.影响寡聚体形成的苏云金芽孢杆菌Cry1Ab突变体对烟草天蛾幼虫无毒。
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Mode of action of mosquitocidal Bacillus thuringiensis toxins.苏云金芽孢杆菌杀蚊毒素的作用模式。
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Bacillus thuringiensis subsp. israelensis Cyt1Aa synergizes Cry11Aa toxin by functioning as a membrane-bound receptor.苏云金芽孢杆菌以色列亚种Cyt1Aa通过作为膜结合受体发挥作用，增强Cry11Aa毒素的活性。
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Cyt1A from Bacillus thuringiensis restores toxicity of Bacillus sphaericus against resistant Culex quinquefasciatus (Diptera: Culicidae).来自苏云金芽孢杆菌的Cyt1A可恢复球形芽孢杆菌对抗性致倦库蚊（双翅目：蚊科）的毒性。
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Cadherin-like receptor binding facilitates proteolytic cleavage of helix alpha-1 in domain I and oligomer pre-pore formation of Bacillus thuringiensis Cry1Ab toxin.类钙黏蛋白受体结合促进了苏云金芽孢杆菌Cry1Ab毒素结构域I中α-1螺旋的蛋白水解切割和寡聚体预孔形成。
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