苏云金芽孢杆菌 Cry11Aa 寡聚化在其对埃及伊蚊的毒性中起重要作用。
Oligomerization of Cry11Aa from Bacillus thuringiensis has an important role in toxicity against Aedes aegypti.
机构信息
Universidad Nacional Autónoma de México, Cuernavaca 62250, Morelos, Mexico.
出版信息
Appl Environ Microbiol. 2009 Dec;75(23):7548-50. doi: 10.1128/AEM.01303-09. Epub 2009 Oct 9.
Abstract
Cry11Aa and Cyt1Aa of Bacillus thuringiensis are active against mosquitoes and show synergism. Cyt1Aa functions as a membrane receptor inducing Cry11Aa oligomerization. Here we characterized Cry11Aa helix alpha-3 mutants impaired in oligomerization and toxicity against Aedes aegypti, indicating that oligomerization of Cry11Aa is important for toxin action. Cyt1Aa did not recover the insecticidal activity of Cry11Aa mutants.
摘要
苏云金芽孢杆菌的 Cry11Aa 和 Cyt1Aa 对蚊子具有活性，并表现出协同作用。Cyt1Aa 作为一种膜受体，诱导 Cry11Aa 寡聚化。在这里，我们对 Cry11Aa 螺旋 α-3 突变体进行了表征，这些突变体在寡聚化和对埃及伊蚊的毒性方面存在缺陷，这表明 Cry11Aa 的寡聚化对于毒素作用很重要。Cyt1Aa 不能恢复 Cry11Aa 突变体的杀虫活性。
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