Chuang L M, Tseng C H, Wu H P, Jou T S, Tai T Y, Lee S C, Lin B J
Department of Internal Medicine, College of Medicine, National Taiwan University Hospital, Taipei, R.O.C.
J Formos Med Assoc. 1990 Nov;89(11):965-8.
The etiology of non-insulin-dependent diabetes mellitus (NIDDM) is still unclear, but appears to involve some genetic factors. There have been disputes over the association between DNA sequences flanking the insulin gene and NIDDM. In order to characterize insulin gene polymorphism in the Chinese population and elucidate its association with NIDDM, 100 unrelated Chinese subjects living in Taiwan were observed for polymorphism of this hypervariable region. Most of them were descendants of immigrants from the southern part of mainland China. Among them, 52 were nondiabetic controls, and 48 were subjects with NIDDM. Insulin gene polymorphism was classified into classes 1, 2 and 3 alleles according to Bell et al. Neither the class 2 allele nor the genotype for the homozygous class 3 allele was not observed in this study. The allelic frequencies of class 1 and 3 genes were 97% and 3% in the nondiabetic subjects, and 99% and 1% in the NIDDM group, respectively. The frequencies of genotypes 1/1 and 1/3 were 94% and 6% in nondiabetics and 98% and 2% in the NIDDM group, respectively. No significant association was found between insulin gene polymorphism and NIDDM. It is concluded that DNA marker flanking the insulin gene may not be associated with the development of NIDDM in Chinese subjects.
非胰岛素依赖型糖尿病（NIDDM）的病因仍不清楚，但似乎涉及一些遗传因素。关于胰岛素基因侧翼的DNA序列与NIDDM之间的关联一直存在争议。为了确定中国人群中胰岛素基因的多态性，并阐明其与NIDDM的关联，对居住在台湾的100名无血缘关系的中国受试者进行了该高变区多态性的观察。他们大多数是中国大陆南部移民的后代。其中，52名是非糖尿病对照，48名是NIDDM患者。根据贝尔等人的方法，胰岛素基因多态性被分为1、2和3类等位基因。在本研究中未观察到2类等位基因和纯合3类等位基因的基因型。非糖尿病受试者中1类和3类基因的等位基因频率分别为97%和3%，NIDDM组分别为99%和1%。非糖尿病患者中1/1和1/3基因型的频率分别为94%和6%，NIDDM组分别为98%和2%。未发现胰岛素基因多态性与NIDDM之间有显著关联。结论是，胰岛素基因侧翼的DNA标记可能与中国受试者NIDDM的发生无关。
