Conn V, Ramsay M, Jenkins T
S Afr Med J. 1987 Feb 7;71(3):174-8.
The hypervariable region 5' to the human insulin gene has been characterised in two South African Indian families, each having two generations of individuals affected with non-insulin-dependent diabetes mellitus (NIDDM). Southern blot analysis, with the restriction endonuclease Pvu II and plasmid phins 310 as a probe, was used. In family 1, class 1 alleles (0.87, 0.79, 0.72 and 0.68 kilobase (kb)) were found at this locus but no linkage with NIDDM was shown. In family 2 a class 3 (2.51 kb) and two class 1 alleles (0.89, 0.76) were found. The 0.89 kb allele appears to be segregating with NIDDM in this family.
人类胰岛素基因5'端的高变区已在两个南非印度裔家庭中得到鉴定，每个家庭都有两代人患有非胰岛素依赖型糖尿病（NIDDM）。使用限制性内切酶Pvu II和质粒phins 310作为探针进行Southern印迹分析。在家庭1中，在该位点发现了1类等位基因（0.87、0.79、0.72和0.68千碱基（kb）），但未显示与NIDDM的连锁关系。在家庭2中，发现了一个3类（2.51 kb）和两个1类等位基因（0.89、0.76）。在这个家庭中，0.89 kb的等位基因似乎与NIDDM共分离。
