Yamasaki Shinji, Asakura Masahiro, Shiramaru Sachi, Inoue Haruna
Graduate School of Life and Environmental Sciences, Osaka Prefecture University.
Nihon Rinsho. 2012 Aug;70(8):1305-12.
Campylobacter jejuni and Campylobacter coli have been leading cause of food poisoning in terms of both number of patients and cases in Japan. Therefore these bacteria are recognized as one of the most important food-borne pathogens. Since campylobacters are microaerobic, slow growing, biochemically unreactive, not only it takes some time but also there are several problems regarding isolation and identification of Campylobacter spp. We found that cytolethal distending toxin (cdt) genes are ubiquitously present in C. jejuni, C. coli and C. fetus in species-specific manner and species-specific multiplex PCRs have been developed on the basis of species-specificity of the cdt genes. In this review, applicability of the cdt gene-based multiplex PCRs as a simple and rapid diagnostic method for clinical and food samples in comparison to the conventional culture methods is described.
空肠弯曲菌和结肠弯曲菌在日本，无论是患者数量还是病例数，都是食物中毒的主要原因。因此，这些细菌被认为是最重要的食源性病原体之一。由于弯曲杆菌是微需氧菌，生长缓慢，生化反应不活跃，不仅分离弯曲杆菌属需要一些时间，而且在分离和鉴定方面还存在几个问题。我们发现细胞致死膨胀毒素（cdt）基因以种属特异性方式普遍存在于空肠弯曲菌、结肠弯曲菌和胎儿弯曲菌中，并基于cdt基因的种属特异性开发了种属特异性多重PCR。在这篇综述中，描述了基于cdt基因的多重PCR作为一种与传统培养方法相比用于临床和食品样本的简单快速诊断方法的适用性。
