Jiangsu University of Science and Technology, Zhenjiang City, Jiangsu Province, 212018, People's Republic of China.
Mol Biol Rep. 2010 Oct;37(7):3171-6. doi: 10.1007/s11033-009-9897-4. Epub 2009 Oct 13.
MicroRNAs (miRNAs) are a class of non-protein coding small RNAs that regulate expression of genes at post-transcriptional levels. Increasing evidence has shown that miRNAs play multiple roles in biological processes, including development, cell proliferation and apoptosis. Based on the conservation of miRNAs sequence, using a computational homology search based on genomic survey sequence analysis, a total of 16 novel miRNAs were identified and characteristics such as family and evolutionary conservation have been described. By using these newly identified miRNAs, the mRNA database of silkworm was blasted and 21 potential targets of miRNAs were detected. Most of these miRNA targeted genes were predicted to encode transcription factors. The semi-stem loop RT-PCR based assays were performed and found that silkworm miRNAs have diverse expression patterns during development.
微小 RNA（miRNAs）是一类非蛋白编码的小 RNA，可在转录后水平调控基因的表达。越来越多的证据表明，miRNAs 在生物过程中发挥多种作用，包括发育、细胞增殖和凋亡。基于 miRNA 序列的保守性，利用基于基因组探测序列分析的计算同源性搜索，共鉴定出 16 个新的 miRNA，并描述了其家族和进化保守性等特征。利用这些新鉴定的 miRNA，对家蚕的 mRNA 数据库进行了轰击，检测到 21 个 miRNA 的潜在靶标。这些 miRNA 靶标基因大多预测编码转录因子。通过半茎环 RT-PCR 检测，发现家蚕 miRNA 在发育过程中具有多样化的表达模式。
