Interdepartmental Research Centre E. Piaggio, University of Pisa, via Diotisalvi 2, Pisa, Italy.
Biotechnol J. 2010 Feb;5(2):232-41. doi: 10.1002/biot.200900074.
Hepatocyte function on 3-D microfabricated polymer scaffolds realised with the pressure-activated microsyringe was tested under static and dynamic conditions. The dynamic cell culture was obtained using the multicompartment modular bioreactor system. Hepatocyte cell density, glucose consumption, and albumin secretion rate were measured daily over a week. Cells seeded on scaffolds showed an increase in cell density compared with monolayer controls. Moreover, in dynamic culture, cell metabolic function increased three times in comparison with static monolayer cultures. These results suggest that cell density and cell-cell interactions are mediated by the architecture of the substrate, while the endogenous biochemical functions are regulated by a sustainable supply of nutrients and interstitial-like flow. Thus, a combination of 3-D scaffolds and dynamic flow conditions are both important for the development of a hepatic tissue model for applications in drug testing and regenerative medicine.
采用压力激活微注射器制造的 3D 微图案聚合物支架上的肝细胞功能在静态和动态条件下进行了测试。动态细胞培养是使用多腔模块式生物反应器系统获得的。在一周的时间内，每天测量肝细胞密度、葡萄糖消耗和白蛋白分泌率。与单层对照相比，接种在支架上的细胞显示出细胞密度增加。此外，在动态培养中，与静态单层培养相比，细胞代谢功能增加了三倍。这些结果表明，细胞密度和细胞间相互作用是由基质的结构介导的，而内源性生化功能是由营养物质的持续供应和类似间质的流动来调节的。因此，3D 支架和动态流动条件的结合对于开发用于药物测试和再生医学的肝组织模型都是重要的。
