Rathbun Kimberly M, Thompson Stuart A
Department of Biochemistry and Molecular Biology, Medical College of Georgia, 1459 Laney-Walker Blvd., Augusta, GA 30912, USA.
FEMS Microbiol Lett. 2009 Nov;300(2):188-94. doi: 10.1111/j.1574-6968.2009.01795.x. Epub 2009 Sep 17.
Campylobacter jejuni is a significant cause of human gastroenteritis worldwide. In an attempt to further define bacterial factors that influence infectivity, Cj0596 was identified as playing a role in C. jejuni virulence. Cj0596 is a periplasmic chaperone that is similar to proteins involved in outer membrane protein (OMP) folding in other bacteria. Mutation of cj0596 caused an alteration in the levels of eight OMPs, compared with wild-type bacteria. Replacement of the cj0596 mutation with the wild-type cj0596 gene restored a wild-type OMP profile. The altered OMP profile in the cj0596 mutant was accompanied by significant changes in several virulence properties, including an increase in the ability to autoagglutinate, increased susceptibility to several antimicrobial agents, and increased biofilm formation. In summary, mutation of cj0596 alters the C. jejuni OMP profile and leads to changes in OMP-related phenotypes involved in C. jejuni pathogenesis.
空肠弯曲菌是全球人类肠胃炎的一个重要病因。为了进一步确定影响感染性的细菌因素，Cj0596被确定在空肠弯曲菌毒力中发挥作用。Cj0596是一种周质伴侣蛋白，与其他细菌中参与外膜蛋白（OMP）折叠的蛋白质相似。与野生型细菌相比，cj0596的突变导致8种OMP水平发生改变。用野生型cj0596基因替换cj0596突变可恢复野生型OMP谱。cj0596突变体中改变的OMP谱伴随着几种毒力特性的显著变化，包括自凝集能力增加、对几种抗菌剂的敏感性增加以及生物膜形成增加。总之，cj0596的突变改变了空肠弯曲菌的OMP谱，并导致空肠弯曲菌发病机制中涉及的OMP相关表型发生变化。
