Zhao Shuo, Ramette Alban, Niu Gui-Lan, Liu Hong, Zhou Ning-Yi
State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
FEMS Microbiol Ecol. 2009 Nov;70(2):159-67. doi: 10.1111/j.1574-6941.2009.00773.x.
Bioaugmentation of nitrobenzene-contaminated soil was performed by inoculation with Pseudomonas putida ZWL73, which can grow on nitrobenzene as carbon and nitrogen sources and release free ammonium from the aromatic ring via a partial-reductive pathway. Removal of nitrobenzene was effectively enhanced with concurrent accumulation of ammonium in the bioaugmented soil. Moreover, the negative impact of nitrobenzene contamination on culturable bacterial types and soil nitrification was reduced by strain ZWL73. Changes in the community structure of ammonia-oxidizing bacteria, determined by denaturing gradient gel electrophoresis, were associated with changes in environmental factors in nitrobenzene-contaminated soil, including concentrations of nitrobenzene, ammonium, nitrite and nitrate, but their influence was attenuated in the bioaugmented soil. Overall, P. putida ZWL73 shows promising abilities for effective removal of nitrobenzene and for attenuating the negative effects of nitrobenzene contamination on soil functioning.
通过接种恶臭假单胞菌ZWL73对受硝基苯污染的土壤进行生物强化，该菌株能够以硝基苯作为碳源和氮源生长，并通过部分还原途径从芳香环释放游离铵。在生物强化土壤中，随着铵的同时积累，硝基苯的去除得到有效增强。此外，菌株ZWL73降低了硝基苯污染对可培养细菌类型和土壤硝化作用的负面影响。通过变性梯度凝胶电泳测定，氨氧化细菌群落结构的变化与硝基苯污染土壤中环境因素的变化有关，包括硝基苯、铵、亚硝酸盐和硝酸盐的浓度，但在生物强化土壤中其影响减弱。总体而言，恶臭假单胞菌ZWL73在有效去除硝基苯以及减轻硝基苯污染对土壤功能的负面影响方面显示出良好的能力。
