Unit of Parasitology, Veterinary Faculty, University of Las Palmas de G.C., Trasmontaña s/n, 35413 Arucas, Las Palmas, Spain.
Vet Parasitol. 2009 Dec 23;166(3-4):228-34. doi: 10.1016/j.vetpar.2009.09.039. Epub 2009 Sep 27.
An ELISA test for the detection of anti-Teladorsagia circumcincta antibodies in goat milk samples (both individual and bulk milk samples) has been developed in dairy goats experimentally infected with a trickle infection. The results observed were compared with those obtained when some other standard methods for the detection of animals infected with gastrointestinal nematodes (GIN), such as faecal egg counts (FEC) and serum pepsinogen levels. Some factors that could affect the final results of the test (dilution and nature of samples as well as their preservation at 4 degrees C for 0-10 days) were also monitored. The results show that the proposed test could allow the detection of infected herds using bulk milk samples. The levels of anti-T. circumcincta antibodies in milk and serum samples and the concentrations of serum pepsinogen showed a similar pattern over time. Refrigeration of milk samples for less than 5 days or its preservation with potassium dichromate for 10 days did not significantly affect the ability of the test to detect infected animals. Finally, when a commercial Ostertagia ostertagi ELISA kit was used to assay milk samples from goats infected with T. circumcincta, the results suggest that it may be possible to use a crude O. ostertagi antigen-based ELISA for the detection of goats infected with T. circumcincta.
一种用于检测羊奶样本（个体和群体奶样）中抗细颈线虫抗体的 ELISA 检测方法已在经微量感染实验感染的乳山羊中开发。将观察到的结果与其他一些用于检测胃肠道线虫（GIN）感染动物的标准方法（如粪便虫卵计数（FEC）和血清胃蛋白酶原水平）进行了比较。还监测了可能影响检测结果的一些因素（稀释度和样本性质以及在 4°C 下保存 0-10 天）。结果表明，该检测方法可用于使用群体奶样检测感染的畜群。随着时间的推移，奶样和血清样本中抗细颈线虫抗体的水平以及血清胃蛋白酶原的浓度呈现出相似的模式。冷藏奶样不超过 5 天或用重铬酸钾保存 10 天不会显著影响检测感染动物的能力。最后，当使用商业的奥斯特氏线虫 ELISA 试剂盒检测感染细颈线虫的山羊的奶样时，结果表明，可能可以使用基于粗奥斯特氏线虫抗原的 ELISA 来检测感染细颈线虫的山羊。
