Kozuch O, Labuda M, Lysý J, Weismann P, Krippel E
Institute of Virology, Slovak Academy of Sciences, Bratislava.
Acta Virol. 1990 Dec;34(6):537-44.
A total of 2922 small terrestrial mammals of 12 species were collected in six localities of West Slovakia between 1981 and 1986. When examined for the prevalence of neutralizing antibodies (NA) to Central European encephalitis (CEE) virus we found that 14.6% had antibody. Nearly all (97%) of the 426 animals with antibody were Clethrionomys glareolus, the most abundant species (52.6% of mammals collected, 15.1% of those with antibody). Apodemus flavicollis (22.5% of mammals collected, 18.1% of those with antibody), Apodemus sylvaticus (14% of mammals collected, 8.5% of those with antibody), and Microtus arvalis (5.5% of mammals collected, 3.3% of those with antibody). In all locations studies the most abundant tick found on small mammals was Ixodes ricinus (larvae and nymphs). Less abundant, but present in all studied sites, were larvae and nymphs of Dermacentor reticulatus and Haemaphysalis concinna. Six strains of CEE virus were isolated from tissues of animals: four from Clethryonomys glareolus and one each from Apodemus flavicollis and Sorex araneus. Three of six isolates were from animals collected in February; none of the six had detectable neutralizing antibody to CEE virus. We discuss these observations with regard to possible mechanisms of persistence of CEE virus.
1981年至1986年间，在斯洛伐克西部的六个地点共采集了2922只12种小型陆生哺乳动物。在检测针对中欧脑炎（CEE）病毒的中和抗体（NA）患病率时，我们发现14.6%的动物有抗体。在426只有抗体的动物中，几乎所有（97%）都是黄颈姬鼠，它是最常见的物种（占采集到的哺乳动物的52.6%，有抗体动物的15.1%）。黄喉姬鼠（占采集到的哺乳动物的22.5%，有抗体动物的18.1%）、林姬鼠（占采集到的哺乳动物的14%，有抗体动物的8.5%）和普通田鼠（占采集到的哺乳动物的5.5%，有抗体动物的3.3%）。在所有研究地点，在小型哺乳动物身上发现的最常见的蜱是蓖麻硬蜱（幼虫和若虫）。虽数量较少但在所有研究地点都存在的是网纹革蜱和全沟硬蜱的幼虫和若虫。从动物组织中分离出了6株CEE病毒：4株来自黄颈姬鼠，1株来自黄喉姬鼠，1株来自蜘蛛猬。6株分离株中有3株来自2月份采集的动物；这6株均未检测到针对CEE病毒的中和抗体。我们就CEE病毒持续存在的可能机制讨论了这些观察结果。
