Rehse-Küpper B, Danielová V, Klenk W, Abar B, Ackermann R
Zentralbl Bakteriol Orig A. 1978 Nov;242(2):148-55.
During the years 1972--1976 a total of 14.692 ticks of the species Ixodes ricinus (L.), (2.738 adults and 11.954 nymphs) was collected. They were taken from five areas where human Central European encephalitis virus (CEE) infections had occurred. By means of, intracerebral (IC) and subcutaneous (SC) isolation experiments with suckling mice 19 agents, exhibiting the pathogenic characteristics of CEE virus, were isolated from these ticks. In neutralization tests with suckling mice these isolates could be identified as CEE virus strains. From the 14.692 ticks at least 1.29% were virus carriers as evidenced by the number of isolated virus strains. By the same reasoning at least 3.6% of the adults and 0.76% of the nymphs must have contained virus. We were successful in each of the five areas, rather distant from each other, to isolate the virus. The favorable ecological conditions for the main vector Ixodes ricinus in the Federal Republic of Germany give rise to the assumption that this medically important tick-borne disease might be permanently present in many areas.
在1972年至1976年期间，共采集到14692只蓖麻硬蜱（Ixodes ricinus (L.)）（2738只成虫和11954只若虫）。这些蜱采自曾发生人类中欧脑炎病毒（CEE）感染的五个地区。通过对乳鼠进行脑内（IC）和皮下（SC）分离实验，从这些蜱中分离出了19种具有CEE病毒致病特征的病原体。在对乳鼠的中和试验中，这些分离株可被鉴定为CEE病毒株。从14692只蜱中分离出的病毒株数量表明，至少1.29%的蜱是病毒携带者。同理，至少3.6%的成虫和0.76%的若虫必定携带病毒。我们在彼此相距甚远的五个地区均成功分离出了病毒。德意志联邦共和国主要病媒蓖麻硬蜱所处的有利生态条件使人推测，这种具有重要医学意义的蜱传疾病可能在许多地区长期存在。
