Laboratory of Cosmetology, Department of Pharmacy, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil.
Skin Res Technol. 2009 Nov;15(4):440-3. doi: 10.1111/j.1600-0846.2009.00386.x.
BACKGROUND/PURPOSE: The continuous advancement in cosmetic science has led to an increasing demand for the development of non-invasive, reliable scientific techniques directed toward claim substantiation, which is of utmost relevance, to obtain data regarding the efficacy and safety of cosmetic products.
In this work, we used the optical coherence tomography (OCT) technique to produce in vitro transversal section-images of human hair. We also compared the OCT signal before and after chemical treatment with an 18% w/w ammonium thioglycolate solution.
The mean diameter of the medulla was 29+/-7 microm and the hair diameter was 122+/-16 microm in our samples of standard Afro-ethnic hair. A three-dimensional (3D) image was constructed starting from 601 cross-sectional images (slices). Each slice was taken in steps of 6.0 microm at eight frames per second, and the entire 3D image was constructed in 60 s.
It was possible to identify, using the A-scan protocol, the principal structures: the cuticle, cortex and medulla. After chemical treatment, it was not possible to identify the main structures of hair fiber due to index matching promoted by deleterious action of the chemical agent.
背景/目的：化妆品科学的不断进步导致人们对开发非侵入性、可靠的科学技术的需求不断增加，这些技术旨在为化妆品产品的功效和安全性提供数据，这一点至关重要。
在这项工作中，我们使用光学相干断层扫描（OCT）技术对人发进行体外横断面成像。我们还比较了 18%w/w 硫代甘氨酸铵溶液化学处理前后的 OCT 信号。
我们的标准非洲裔人发样本的中髓直径平均值为 29+/-7 微米，头发直径为 122+/-16 微米。从 601 个横截面图像（切片）构建了一个三维（3D）图像。每个切片以 6.0 微米的步长在每秒 8 帧的速度下拍摄，整个 3D 图像在 60 秒内构建完成。
使用 A 扫描协议，可以识别主要结构：表皮、皮质和髓质。化学处理后，由于化学试剂的有害作用导致折射率匹配，无法识别毛发纤维的主要结构。
