Johnson & Johnson Consumer and Personal Products Worldwide, Skillman, NJ 08558, USA.
Skin Res Technol. 2009 Nov;15(4):482-8. doi: 10.1111/j.1600-0846.2009.00394.x.
BACKGROUND/PURPOSE: The ability to optically section live biological tissue in vivo with laser light is made possible by confocal laser scanning microscopy (CLSM). In this work, the effects of changing the wavelength of incident light used for CLSM imaging of human skin are reported and analyzed.
Optical phantoms and the skin of eight human volunteers were imaged using CLSM systems having three different incident light wavelengths (405, 785, and 830 nm).
Qualitative and quantitative differences were observed between images obtained at each wavelength, despite the proximity of the two near infrared 785 and 830 nm wavelengths. Furthermore, the penetration depth achieved with the 405 nm CLSM permitted imaging into the papillary dermis.
The laser wavelength used in CLSM reflectance imaging is important to properly understand and resolve different biological structures within human skin.
背景/目的：共聚焦激光扫描显微镜（CLSM）使得利用激光对活生物组织进行光学切片成为可能。在这项工作中，报告并分析了改变用于 CLSM 对人皮肤成像的入射光波长的效果。
使用具有三种不同入射光波长（405、785 和 830nm）的 CLSM 系统对光学模型和 8 名志愿者的皮肤进行成像。
尽管近红外 785nm 和 830nm 两个波长非常接近，但在每个波长获得的图像之间观察到了定性和定量的差异。此外，405nmCLSM 实现的穿透深度允许对乳头真皮进行成像。
CLSM 反射成像中使用的激光波长对于正确理解和解析人皮肤内的不同生物结构非常重要。
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