Sakamoto A, Takeba G, Shibata D, Tanaka K
Faculty of Agriculture, Kyoto Prefectural University, Shimogamo, Japan.
Plant Mol Biol. 1990 Aug;15(2):317-23. doi: 10.1007/BF00036917.
A full-length cDNA encoding glutamine synthetase was isolated from a lambda gt11 library constructed from the poly(A)+ RNA isolated from lettuce seeds incubated under red light. The nucleotide sequence of the cDNA and the deduced sequence of amino acids showed a high degree of homology to those of the cytosol-type glutamine synthetase from other plants. Northern and dot-blot analyses of poly(A)+ RNA extracted from the seeds incubated under various light conditions showed that the activation of the gene for cytosolic glutamine-synthetase during imbibition of lettuce seeds is directly or indirectly regulated by phytochrome.
从用红光处理的生菜种子中分离出的poly(A)+ RNA构建的λgt11文库中，分离出了一个编码谷氨酰胺合成酶的全长cDNA。该cDNA的核苷酸序列和推导的氨基酸序列与其他植物的胞质型谷氨酰胺合成酶的序列具有高度同源性。对在各种光照条件下处理的种子中提取的poly(A)+ RNA进行Northern印迹和斑点印迹分析表明，生菜种子吸胀期间胞质谷氨酰胺合成酶基因的激活直接或间接受光敏色素调控。
