Friedrich Mike, Nozadze Revaz, Gan Qiang, Zelman-Femiak Monika, Ermolayev Vladimir, Wagner Toni U, Harms Gregory S
Molecular Microscopy Group, Rudolf Virchow Center, University of Würzburg, Versbacher Str 9, D-97078 Würzburg, Germany.
Biochem Biophys Res Commun. 2009 Dec 18;390(3):722-7. doi: 10.1016/j.bbrc.2009.10.036. Epub 2009 Oct 13.
Single-molecule detection and tracking is important for observing biomolecule interactions in the microenvironment. Here we report selective plane illumination microscopy (SPIM) with single-molecule detection in living organisms, which enables fast imaging and single-molecule tracking and optical penetration beyond 300 microm. We detected single nanocrystals in Drosophila larvae and zebrafish embryo. We also report our first tracking of single quantum dots during zebrafish development, which displays a transition from flow to confined motion prior to the blastula stage. The new SPIM setup represents a new technique, which enables fast single-molecule imaging and tracking in living systems.
单分子检测与追踪对于观察微环境中的生物分子相互作用至关重要。在此，我们报告了一种用于活生物体中单分子检测的选择性平面照明显微镜（SPIM），它能够实现快速成像、单分子追踪以及超过300微米的光学穿透。我们在果蝇幼虫和斑马鱼胚胎中检测到了单个纳米晶体。我们还报告了在斑马鱼发育过程中对单个量子点的首次追踪，其显示在囊胚期之前从流动到受限运动的转变。新的SPIM装置代表了一种新技术，能够在活体系统中实现快速单分子成像与追踪。
