光学断层扫描技术可辅助光片显微镜对斑马鱼发育进行整体成像。

Bassi Andrea, Schmid Benjamin, Huisken Jan

Max Planck Institute of Molecular Cell Biology and Genetics, Dresden 01307, Germany Politecnico di Milano, Dipartimento di Fisica, Milano 20133, Italy.

Max Planck Institute of Molecular Cell Biology and Genetics, Dresden 01307, Germany.

Development. 2015 Mar 1;142(5):1016-20. doi: 10.1242/dev.116970.

Fluorescently labeled structures can be spectrally isolated and imaged at high resolution in living embryos by light sheet microscopy. Multimodal imaging techniques are now needed to put these distinct structures back into the context of the surrounding tissue. We found that the bright-field contrast of unstained specimens in a selective plane illumination microscopy (SPIM) setup can be exploited for in vivo tomographic reconstructions of the three-dimensional anatomy of zebrafish, without causing phototoxicity. We report multimodal imaging of entire zebrafish embryos over several hours of development, as well as segmentation, tracking and automatic registration of individual organs.

通过光片显微镜技术，可在活胚胎中对荧光标记结构进行光谱分离并以高分辨率成像。现在需要多模态成像技术将这些不同的结构放回周围组织的背景中。我们发现，在选择性平面照明显微镜（SPIM）设置中，未染色标本的明场对比度可用于斑马鱼三维解剖结构的体内断层重建，且不会产生光毒性。我们报告了对整个斑马鱼胚胎在数小时发育过程中的多模态成像，以及对单个器官的分割、跟踪和自动配准。