Key Laboratory of Microbial Metabolism, Ministry of Education, College of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, People's Republic of China.
Pest Manag Sci. 2010 Mar;66(3):248-52. doi: 10.1002/ps.1856.
Pyoluteorin (Plt) is a polyketide metabolite produced by fluorescent Pseudomonas. It controls a wide range of pests, including bacteria, epiphytes and weeds. It could become widely used as a new biological pesticide. However, ignorance of the stability of Plt is an obstacle to its use. This work studied the stability of Plt and its degradation under different conditions, including temperature, pH and UV-visible light irradiation.
Degradation of Plt followed first-order reaction kinetics. The degradation rate increased with increased temperature. The derived activation energy was 11.06 J mol(-1) K(-1). Plt was relatively stable in neutral solutions, in contrast to acid and alkaline solutions. Visible irradiation had no obvious effect on Plt stability, while UV irradiation greatly decreased its half-life to 3-4 days, in contrast to its half-life of 25 days in the dark.
Plt is relatively stable in pure water solutions and at room temperature, with a half-life of more than 20 days. However, UV irradiation and acidic or alkaline solutions will enhance its degradation, reducing its half-life by a factor of 0.1-0.3. Before Plt is widely used as a pesticide, it might be necessary to modify the structure of Plt to make it more stable.
吡咯并喹啉醌（Plt）是荧光假单胞菌产生的一种聚酮代谢物。它能控制包括细菌、附生植物和杂草在内的多种害虫，有望成为一种新型生物农药得到广泛应用。然而，由于人们对其稳定性认识不足，阻碍了其应用。本研究考察了 Plt 在不同条件下的稳定性及其降解情况，包括温度、pH 值和紫外-可见光照。
Plt 的降解遵循一级反应动力学。降解速率随温度升高而增加，其活化能为 11.06 J mol(-1) K(-1)。Plt 在中性溶液中相对稳定，而在酸性和碱性溶液中则不稳定。可见光照射对 Plt 稳定性没有明显影响，而紫外光照射使其半衰期大大缩短至 3-4 天，而在黑暗中半衰期为 25 天。
Plt 在纯水溶液和室温下相对稳定，半衰期超过 20 天。然而，紫外光照射和酸性或碱性溶液会加速其降解，使其半衰期缩短至 0.1-0.3 倍。在广泛用作农药之前，可能需要对 Plt 的结构进行修饰，以提高其稳定性。
