Stashenko Elena E, Andrés Ordóñez Sergio, Marín Néstor Armando, Martínez Jairo René
Research Center of Excellence CENIVAM, Chromatography Laboratory, CIBIMOL, Universidad Industrial de Santander. Carrera 27 Calle 9. Bucaramanga, Colombia.
J Chromatogr Sci. 2009 Oct;47(9):817-21. doi: 10.1093/chromsci/47.9.817.
Volatile and semi-volatile secondary metabolites, as well as aristolochic acids (AA), present in leaves, stems, and flowers of Aristolochia ringens were determined by gas chromatography (GC)-mass spectrometry (MS) and high-performance liquid chromatography (HPLC) methods, respectively. Metabolite isolation was performed using different extraction techniques: microwave-assisted hydrodistillation (MWHD), supercritical fluid extraction, and headspace solid-phase microextraction (HS-SPME). The chemical composition of the extracts and oils was established by GC-MS. The determinations of AAI and AAII were conducted by methanolic extraction of different plant parts followed by HPLC analysis. Essential oil yields from leaves and stems were 0.008 +/- 0.0022% and 0.047 +/- 0.0026%, respectively. Aristolochia ringens flowers did not yield essential oil under MWHD. Sesquiterpene hydrocarbons (66%) were the main compounds in the essential oil isolated from leaves whereas monoterpene hydrocarbons (73%) predominated in the stems essential oil. Yields of extracts isolated by SFE from leaves, stems, and flowers were 4 +/- 1.8%, 1.2 +/- 0.25%, and 4 +/- 1.8%, respectively. In vivo HS-SPME of flowers isolated compounds with known unpleasant smells such as volatile aldehydes and short-chain carboxylic acids. HPLC analysis detected the presence of AAII in the flowers of Aristolochia ringens at a concentration of 610 +/- 47 mg/kg of dried flower.
采用气相色谱（GC）-质谱（MS）法和高效液相色谱（HPLC）法分别测定了马兜铃叶、茎和花中挥发性和半挥发性次生代谢产物以及马兜铃酸（AA）。使用不同的提取技术进行代谢物分离：微波辅助水蒸馏（MWHD）、超临界流体萃取和顶空固相微萃取（HS-SPME）。通过GC-MS确定提取物和油的化学成分。通过对不同植物部位进行甲醇提取然后进行HPLC分析来测定AAI和AAII。叶和茎的精油产量分别为0.008±0.0022%和0.047±0.0026%。在MWHD条件下，马兜铃花未产生精油。倍半萜碳氢化合物（66%）是从叶中分离出的精油中的主要成分，而单萜碳氢化合物（73%）在茎精油中占主导地位。通过超临界流体萃取（SFE）从叶、茎和花中分离出的提取物产量分别为4±1.8%、1.2±0.25%和4±1.8%。花的体内HS-SPME分离出了具有难闻气味的已知化合物，如挥发性醛和短链羧酸。HPLC分析检测到马兜铃花中存在AAII，其浓度为610±47mg/kg干花。
