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结构研究免疫增强水溶性葡聚糖从食用蘑菇热水提取物中分离出来,佛罗里达香菇,品种阿萨姆邦佛罗里达。

Structural studies of an immunoenhancing water-soluble glucan isolated from hot water extract of an edible mushroom, Pleurotus florida, cultivar Assam Florida.

机构信息

Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721 102, West Bengal, India.

出版信息

Carbohydr Res. 2009 Dec 14;344(18):2596-601. doi: 10.1016/j.carres.2009.09.010. Epub 2009 Sep 29.

DOI:10.1016/j.carres.2009.09.010
PMID:19836727
Abstract

An immunoenhancing polysaccharide isolated from the hot water extract of the fruiting bodies of an edible mushroom Pleurotus florida, cultivar Assam Florida, was found to consist of only d-glucose as a monosaccharide constituent. On the basis of total acid hydrolysis, methylation analysis, periodate oxidation, Smith degradation, and NMR experiments ((1)H, (13)C, DEPT-135, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC), the structure of the repeating unit of the polysaccharide was established as This glucan stimulates macrophages, splenocytes, and thymocytes.

摘要

从可食用蘑菇佛罗里达侧耳(Pleurotus florida)的子实体热水提取物中分离出的一种免疫增强多糖,经鉴定仅由 d-葡萄糖作为单糖组成。根据完全酸水解、甲基化分析、高碘酸氧化、Smith 降解和 NMR 实验(1H、13C、DEPT-135、DQF-COSY、TOCSY、NOESY、ROESY、HMQC 和 HMBC),多糖重复单元的结构确定为该葡聚糖可刺激巨噬细胞、脾细胞和胸腺细胞。

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