Department of Pharmacology, Shri Ram Institute of Technology-Pharmacy, Jabalpur, Madhya Pradesh, India.
Indian J Pharmacol. 2011 Nov;43(6):667-70. doi: 10.4103/0253-7613.89823.
The aim of the present work was to evaluate the in vitro effect of Pleurotus florida extract cataract induced by glucose.
Goat eye lenses were divided into four groups. Group I lenses were incubated in artificial aqueous humor with glucose concentration 5.5 mM (normal control). Group II lenses were incubated with glucose concentration 55 mM (toxic control). Group III and IV lenses incubated with glucose concentration 55 mM were incubated along with hydroethanolic extract of P. florida 250 μg/ml and 500 μg/ml and subjected to morphological and biochemical evaluation.
Group II lenses showed high amount of malondialdehyde (MDA) soluble and insoluble protein and decreased catalase and glutathione levels, while lenses treated with P. florida extract showed significant (P < 0.05) reduction in MDA, increased level of catalase (P < 0.001), glutathione (P < 0.005) and total and soluble protein.
Hydroethanolic extract of P. florida showed prevention of in vitro glucose induced cataract. Thus, the goat lens model could be used for testing of various anticataract agents.
本研究旨在评估佛罗里达侧耳（Pleurotus florida）提取物对葡萄糖诱导白内障的体外作用。
将山羊晶状体分为四组。第 I 组晶状体在含有 5.5mM 葡萄糖的人工房水中孵育（正常对照组）。第 II 组晶状体在含有 55mM 葡萄糖的溶液中孵育（毒性对照组）。第 III 和 IV 组晶状体在含有 55mM 葡萄糖的溶液中孵育，并分别加入 250μg/ml 和 500μg/ml 的佛罗里达侧耳水醇提取物，进行形态学和生化评估。
第 II 组晶状体中丙二醛（MDA）、可溶性和不溶性蛋白质含量较高，过氧化氢酶和谷胱甘肽水平降低，而用佛罗里达侧耳提取物处理的晶状体中 MDA 显著减少（P < 0.05），过氧化氢酶（P < 0.001）、谷胱甘肽（P < 0.005）和总蛋白及可溶性蛋白水平升高。
佛罗里达侧耳水醇提取物可预防体外葡萄糖诱导的白内障。因此，山羊晶状体模型可用于测试各种抗白内障药物。
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