Section on Physical Biochemistry, Laboratory of Biochemistry and Genetics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, U.S. Department of Health and Human Services, Bethesda, MD 20892, USA.
Anal Biochem. 2010 Feb 15;397(2):247-9. doi: 10.1016/j.ab.2009.10.023. Epub 2009 Nov 1.
When peripheral proteins bind to phospholipid membranes lacking discrete binding sites, steric repulsion between bound protein molecules may result in a reduction of the surface area available to additional bound protein by an amount significantly greater than the actual area occupied by bound protein. An approximate treatment of this effect demonstrates that neglect of area exclusion by bound protein may lead to significant errors in the evaluation of equilibrium association constants and the fractional coverage of membrane surface area.
当外周蛋白与缺乏离散结合位点的磷脂膜结合时，结合蛋白分子之间的空间排斥可能导致可用于额外结合蛋白的表面积减少，其减少量明显大于结合蛋白实际占据的面积。对这种效应的近似处理表明，忽略结合蛋白的面积排除可能导致在评估平衡缔合常数和膜表面积的分数覆盖时产生显著误差。
