Genomics Research Center, Academia Sinica, Taipei 115, Taiwan.
Bioorg Med Chem. 2009 Nov 15;17(22):7831-40. doi: 10.1016/j.bmc.2009.09.002. Epub 2009 Sep 6.
Using Caenorhabditis elegans as a model organism, various natural substances and commercial health-food supplements were screened to evaluate their effects on longevity. Among the substances tested, acetic acid and Reishi polysaccharide fraction 3 (RF3) were shown to increase the expression of the lifespan and longevity-related transcription factor DAF-16 in C. elegans. We have shown that RF3 activates DAF-16 expression via TIR-1 receptor and MAPK pathway whereas acetic acid inhibits the trans-membrane receptor DAF-2 of the insulin/IGF-1 pathway to indirectly activate DAF-16 expression. In addition, a mixture of acetic acid and RF3 possesses a combined effect 30-40% greater than either substance used alone. A proteomic analysis of C. elegans using 2-DE and LC-MS/MS was then carried out, and 15 differentially expressed proteins involved in the lifespan-promoting activity were identified.
利用秀丽隐杆线虫作为模式生物，筛选了各种天然物质和商业保健品，以评估它们对寿命的影响。在测试的物质中，醋酸和灵芝多糖 3 （RF3）被证明能增加线虫中寿命和与寿命相关的转录因子 DAF-16 的表达。我们已经表明，RF3 通过 TIR-1 受体和 MAPK 途径激活 DAF-16 的表达，而醋酸通过抑制胰岛素/IGF-1 途径的跨膜受体 DAF-2 间接激活 DAF-16 的表达。此外，醋酸和 RF3 的混合物具有比单独使用任何一种物质强 30-40%的协同作用。然后对秀丽隐杆线虫进行了 2-DE 和 LC-MS/MS 的蛋白质组学分析，鉴定出 15 种参与寿命促进活性的差异表达蛋白。
