Yang Yan-Jie
College of Animal Science and Technology, Northwest A & F University, Yangling 712100, China.
Yi Chuan. 2009 Oct;31(10):1006-12. doi: 10.3724/sp.j.1005.2009.01006.
Four hundred and five Qinchuan cattle at the age of 24 months were used to detect SNPs of adiponectin gene by PCR-SSCP and sequencing technology and to analyze the correlation of SNPs with carcass and meat quality traits using the general linear model (GLM) in SPSS program. Five genotypes (AA, AB, BB, CC, CD) were detected,with one G-->C mutation at 64 bp in exon2 of adiponectin in ABBB genotypes and one C-->T mutation at 50 bp in exon3 of adiponectin in CD genotype. G-->C mutation resulted glutamic acid (GGA) into glutamine (GCA) and C-->T mutation resulted serine (TCA) into leucine (TTA). Statistical analysis revealed that Qinchuan cattle with AA genotype was higher than BB genotype in slaughter weight, back fat thickness, carcass weight, loin muscle area (P < 0.05). The crural girth of AA genotype was significantly higher than AB and BB genotypes (P < 0.01). Qinchuan cattle with CD genotype was higher than CC genotype in slaughter weight, subcutaneous fat thickness, back fat thickness, crural girth, and tenderness (P < 0.05). Adiponectin gene was proved to be closely related to carcass and meat quality traits (P < 0.05), which can be used as a candidate molecular marker for production of high-grade meat in Qinchuan beef cattle.
选用405头24月龄的秦川牛，采用PCR-SSCP和测序技术检测脂联素基因的单核苷酸多态性（SNPs），并运用SPSS软件中的一般线性模型（GLM）分析SNPs与胴体和肉质性状的相关性。共检测到5种基因型（AA、AB、BB、CC、CD），其中ABBB基因型在脂联素基因外显子2的64 bp处发生了1个G→C突变，CD基因型在脂联素基因外显子3的50 bp处发生了1个C→T突变。G→C突变导致谷氨酸（GGA）变为谷氨酰胺（GCA），C→T突变导致丝氨酸（TCA）变为亮氨酸（TTA）。统计分析表明，AA基因型秦川牛的屠宰体重、背膘厚、胴体重、腰大肌面积均高于BB基因型（P<0.05）。AA基因型的胸围显著高于AB和BB基因型（P<0.01）。CD基因型秦川牛的屠宰体重、皮下脂肪厚度、背膘厚、胸围和嫩度均高于CC基因型（P<0.05）。结果证明脂联素基因与胴体和肉质性状密切相关（P<0.05），可作为秦川肉牛高档肉生产的候选分子标记。
