Han Rui-Hua, Zan Lin-Sen, Yang Da-Peng, Hao Rong-Chao
College of Animal Science and Technology, Northwest Aamp;F University, Yangling 712100, China.
Yi Chuan. 2008 Dec;30(12):1579-84. doi: 10.3724/sp.j.1005.2008.01579.
PCR-SSCP technology was used to analyze the correlation of polymorphisms IGF2 (Insulin-like growth factor) gene with carcass and meat quality traits in 186 Qinchuan cattle at the age of 24-month. C-->T mutation in 120 and A-->G mutation in 279 of IGF2 gene. Statistical analysis indicated that Qinchuan cattle with genotype BB and DD had significant differences in slaughter weight, carcass weight, carcass length, carcass chest depth and eye muscle area (P<0.05). The difference in thickness of back fat was significant (P<0.01). Significant differences in marbling, tenderness, pH24 of meat quality (P<0.05), but the differences in carcass depth and water holding capacity were not significant (P>0.05). Genotype AA, DD were predominant genotypes and A, D were predominant alleles. The population containing B, D allele had more excellent carcass and meat quality than others, especially in the capacity of fat accumulate.
采用聚合酶链反应-单链构象多态性（PCR-SSCP）技术，对186头24月龄秦川牛胰岛素样生长因子2（IGF2）基因多态性与胴体及肉质性状的相关性进行分析。IGF2基因第120位发生C→T突变，第279位发生A→G突变。统计分析表明，基因型为BB和DD的秦川牛在屠宰体重、胴体重量、胴体长度、胴体胸深和眼肌面积方面存在显著差异（P<0.05）。背膘厚度差异极显著（P<0.01）。肉质的大理石花纹、嫩度、pH24值存在显著差异（P<0.05），但胴体深度和系水力差异不显著（P>0.05）。基因型AA、DD为优势基因型，A、D为优势等位基因。含有B、D等位基因的群体比其他群体具有更优良的胴体和肉质性状，尤其是在脂肪沉积能力方面。
