Laboratory of Chemical Biotechnology, Faculty of Biochemical and Chemical Engineering, TU Dortmund, Emil-Figge-Strasse 66, 44221 Dortmund, Germany.
Biotechnol Bioeng. 2010 Mar 1;105(4):705-17. doi: 10.1002/bit.22584.
Biofilms are known to be robust biocatalysts. Conventionally, they have been mainly applied for wastewater treatment, however recent reports about their employment for chemical synthesis are increasingly attracting attention. Engineered Pseudomonas sp. strain VLB120 Delta C biofilm growing in a tubular membrane reactor was utilized for the continuous production of (S)-styrene oxide. A biofilm specific morphotype appeared in the effluent during cultivation, accounting for 60-80% of the total biofilm irrespective of inoculation conditions but with similar specific activities as the original morphotype. Mass transfer of the substrate styrene and the product styrene oxide was found to be dependent on the flow rate but was not limiting the epoxidation rate. Oxygen was identified as one of the main parameters influencing the biotransformation rate. Productivity was linearly dependent on the specific membrane area and on the tube wall thickness. On average volumetric productivities of 24 g L(aq) (-1) day(-1) with a maximum of 70 g L(aq) (-1) day(-1) and biomass concentrations of 45 g (BDW) L(aq) (-1) have been achieved over long continuous process periods (>or=50 days) without reactor downtimes.
生物膜被认为是强大的生物催化剂。传统上，它们主要应用于废水处理，然而，最近关于它们在化学合成中的应用的报道越来越受到关注。在管状膜反应器中生长的工程假单胞菌 VLB120 Delta C 生物膜被用于（S）-氧化苯乙烯的连续生产。在培养过程中，在流出物中出现了一种特定的生物膜形态，占总生物膜的 60-80%，而与接种条件无关，但与原始形态的比活性相似。发现底物苯乙烯和产物苯乙烯氧化物的传质取决于流速，但不限制环氧化速率。氧被确定为影响生物转化速率的主要参数之一。生产力与比膜面积和管壁厚度呈线性相关。在长连续过程（>或=50 天）期间，无需停机，平均体积产率为 24 g L(aq) (-1) day(-1)，最高可达 70 g L(aq) (-1) day(-1)，生物量浓度为 45 g (BDW) L(aq) (-1)。
