Poole C A, Matsuoka A, Schofield J R
Department of Surgery, University of Auckland School of Medicine, New Zealand.
Arthritis Rheum. 1991 Jan;34(1):22-35. doi: 10.1002/art.1780340105.
Chondrons were isolated from human and canine osteoarthritic cartilage using low-speed homogenization techniques. Changes in chondron morphology were evaluated using differential interference-contrast microscopy, phase-contrast microscopy, and histochemical and ultrastructural methods. Chondrocyte viability was assessed using fluorescein diacetate staining, and chondron metabolism was investigated using autoradiography. The results suggest that initial changes in the collagen and proteoglycan distribution within the chondron are followed by chondrocyte proliferation to form clusters. These techniques offer the potential to study cell matrix interactions in degenerative osteoarthritis.
使用低速匀浆技术从人和犬的骨关节炎软骨中分离出软骨粒。采用微分干涉相差显微镜、相差显微镜以及组织化学和超微结构方法评估软骨粒形态的变化。使用荧光素二乙酸酯染色评估软骨细胞活力，并通过放射自显影研究软骨粒代谢。结果表明，软骨粒内胶原蛋白和蛋白聚糖分布的初始变化之后是软骨细胞增殖形成簇。这些技术为研究退行性骨关节炎中的细胞-基质相互作用提供了可能。
