Poole C A, Flint M H, Beaumont B W
Department of Surgery, University of Auckland Medical School, New Zealand.
J Orthop Res. 1988;6(3):408-19. doi: 10.1002/jor.1100060312.
We report on the morphology and structure of single and multiple chondrons isolated from homogenized samples of fresh and fixed canine tibial cartilage. Phase contrast, Nomarski, and scanning electron microscopy observations show each chondron to be composed of a chondrocyte and its pericellular matrix enclosed within a "felt-like" pericellular capsule. The extraction of intact chondrons from cartilage homogenates confirms the structural validity of the chondron concept and emphasizes the intrinsic mechanical strength of the capsule. Frayed collagen fibers radiate from multiple chondron columns suggesting a shear-resistant, structural interrelationship between capsular components and type II collagen fibers. Future development of chondron extraction procedures could provide a unique model with which to study the structure, biochemistry, and function of articular cartilage chondrocytes and their pericellular microenvironment.
我们报告了从新鲜和固定犬胫骨软骨匀浆样本中分离出的单个和多个软骨粒的形态和结构。相差显微镜、诺马斯基显微镜和扫描电子显微镜观察表明，每个软骨粒由一个软骨细胞及其周围基质组成，周围基质被包裹在一个“毡状”的细胞周囊中。从软骨匀浆中提取完整的软骨粒证实了软骨粒概念的结构有效性，并强调了囊的内在机械强度。多条软骨粒柱放射出磨损的胶原纤维，表明囊成分与II型胶原纤维之间存在抗剪切的结构相互关系。软骨粒提取程序的未来发展可能会提供一个独特的模型，用于研究关节软骨软骨细胞及其周围微环境的结构、生物化学和功能。
