EKLF 缺失小鼠血液中的巨核细胞-红细胞谱系混杂。
Megakaryocyte-erythroid lineage promiscuity in EKLF null mouse blood.
机构信息
Institute for Molecular Bioscience, University of Queensland, St Lucia, 4072 Qld, Australia.
出版信息
Haematologica. 2010 Jan;95(1):144-7. doi: 10.3324/haematol.2009.010017. Epub 2009 Oct 22.
Abstract
Commitment towards megakaryocyte versus erythroid blood cell lineages occurs in the megakaryocyte-erythroid progenitor, where mutually exclusive expression of either EKLF (Klf1) or Fli1 defines alternative outcomes. Here we show there is a marked increase in the number of circulating platelets in mice lacking the erythroid transcription factor EKLF. In addition, committed erythroid cells retain key signatures of megakaryocytes both on the cell surface and at the mRNA level. We also show that the effect of EKLF on megakaryocyte-erythroid progenitor lineage decision and commitment is cell autonomous in bone marrow reconstitution assays where stem cells lacking EKLF favor the megakaryocyte differentiation pathway. We conclude the megakaryocyte program is aberrantly activated in EKLF null erythroid cells.
摘要
向巨核细胞与红细胞谱系的分化发生在巨核细胞-红细胞祖细胞中，在这里，EKLF（Klf1）或 Fli1 的相互排斥表达决定了不同的结果。在这里，我们发现缺乏红细胞转录因子 EKLF 的小鼠循环血小板数量显著增加。此外，在骨髓重建成分实验中，定向的红细胞细胞在细胞表面和 mRNA 水平上保留了巨核细胞的关键特征。我们还表明，EKLF 对巨核细胞-红细胞祖细胞谱系决定和定向的影响在骨髓重建成分实验中是细胞自主的，其中缺乏 EKLF 的干细胞偏向巨核细胞分化途径。我们得出结论，巨核细胞程序在 EKLF 缺失的红细胞中异常激活。
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