对来自詹氏甲烷球菌DSM 2661的一种假定蛋白MJ0754进行克隆、纯化及初步X射线晶体学分析。
Cloning, purification and preliminary X-ray crystallographic analysis of a hypothetical protein, MJ0754, from Methanococcus jannaschii DSM 2661.
作者信息
Lee Eun Hye, Nam Ki Hyun, Hwang Kwang Yeon
机构信息
Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Republic of Korea.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Oct 1;65(Pt 10):1065-7. doi: 10.1107/S1744309109036562. Epub 2009 Sep 25.
The protein encoded by the MJ0754 gene from the archaeon Methanococcus jannaschii DSM 2661 is an unknown hypothetical protein. Two recombinant proteins, MJ0754 (residues 1-185) and MJ0754t (a truncated form of MJ0754, residues 11-185), were cloned from MJ0754, overexpressed as His-tag fusion proteins and purified. The crystals were found to grow under two different conditions and to have two different shapes. The crystal of MJ0754 belonged to space group P6(1), with unit-cell parameters a = b = 127.015, c = 48.929 A, a calculated Matthews coefficient of 2.85 A(3) Da(-1) and two molecules per asymmetric unit. The crystal of MJ0754t belonged to space group C222(1), with unit-cell parameters a = 51.915, b = 79.122, c = 93.869 A, a calculated Matthews coefficient of 2.41 A(3) Da(-1) and one molecule per asymmetric unit. The SeMet-labelled P6(1) crystal diffracted to a resolution of 3.1 A, while the native C222(1) crystal diffracted to 1.3 A resolution.
来自嗜热栖热甲烷球菌DSM 2661的MJ0754基因编码的蛋白质是一种未知的假设蛋白。从MJ0754中克隆出两种重组蛋白，MJ0754（第1 - 185位氨基酸残基）和MJ0754t（MJ0754的截短形式，第11 - 185位氨基酸残基），将它们作为His标签融合蛋白进行过表达并纯化。发现晶体在两种不同条件下生长且具有两种不同形状。MJ0754的晶体属于空间群P6(1)，晶胞参数a = b = 127.015，c = 48.929 Å，计算得到的马修斯系数为2.85 ų Da⁻¹，每个不对称单位中有两个分子。MJ0754t的晶体属于空间群C222(1)，晶胞参数a = 51.915，b = 79.122，c = 93.869 Å，计算得到的马修斯系数为2.41 ų Da⁻¹，每个不对称单位中有一个分子。硒代甲硫氨酸标记的P6(1)晶体衍射分辨率为3.1 Å，而天然的C222(1)晶体衍射分辨率为1.3 Å。
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