Department of Anesthesia and Perioperative Medicine, Kobe University Hospital Anesthesiology, Kobe, Japan.
Tohoku J Exp Med. 2009 Nov;219(3):215-22. doi: 10.1620/tjem.219.215.
Hyperglycemia amplifies the inflammatory state after ischemia/reperfusion (I/R), and activated neutrophils have been implicated in the development of I/R-induced renal injuries. D-ribose is a naturally occurring monosaccharide found in all living cells. In this study, we examined whether D-ribose attenuates I/R-induced renal injury by reducing neutrophil activation in rats with transient hyperglycemia. Male Wistar rats were divided into sham (n = 24), control (n = 64), and D-ribose (n = 32) groups. Rats received intraperitoneal injection of glucose (3 g/kg) 30 min before induction of ischemia to induce transient hyperglycemia. Anesthetized rats underwent right nephrectomy and subsequent occlusion of the left renal artery and vein for 45 min. D-ribose (400 mg/kg) was intravenously administered 30 min before induction of ischemia. D-ribose significantly reduced the degree of the I/R-induced increases in renal concentrations of cytokine-induced neutrophil chemoattractant-1 (a chemotactic factor for the activation of neutrophils and chemotaxis to the site of injury) and myeloperoxidase (an indicator of neutrophils infiltration). D-ribose also reduced the I/R-induced increases in serum levels of blood urea nitrogen and creatinine, and improved histological changes, including acute tubular necrosis in the corticomedullary junction fields. These results indicate that D-ribose reduces the I/R-induced acute renal injury in rats with transient hyperglycemia, probably by reducing neutrophil activation. D-ribose might thus be useful for surgical procedures, such as renal transplant surgery, under hyperglycemia.
高血糖会放大缺血/再灌注（I/R）后的炎症状态，而激活的中性粒细胞被认为与 I/R 引起的肾损伤的发展有关。D-核糖是一种存在于所有活细胞中的天然单糖。在这项研究中，我们研究了 D-核糖是否通过减少短暂高血糖大鼠中性粒细胞的激活来减轻 I/R 引起的肾损伤。雄性 Wistar 大鼠分为假手术（n = 24）、对照组（n = 64）和 D-核糖组（n = 32）。大鼠在诱导缺血前 30 分钟腹腔注射葡萄糖（3 g/kg）以诱导短暂高血糖。麻醉大鼠行右肾切除术，随后左肾动静脉闭塞 45 分钟。D-核糖（400 mg/kg）在诱导缺血前 30 分钟静脉给药。D-核糖显著降低了 I/R 诱导的肾组织细胞因子诱导的中性粒细胞趋化因子-1（一种激活中性粒细胞的趋化因子和向损伤部位趋化的趋化因子）和髓过氧化物酶（中性粒细胞浸润的指标）水平升高的程度。D-核糖还降低了 I/R 诱导的血清血尿素氮和肌酐水平的升高，并改善了组织学变化，包括皮质髓质交界处的急性肾小管坏死。这些结果表明，D-核糖通过减少中性粒细胞的激活，降低了短暂高血糖大鼠的 I/R 诱导的急性肾损伤。因此，D-核糖可能对高血糖下的手术过程（如肾移植手术）有用。
