Chimma Pattamawan, Roussilhon Christian, Sratongno Panudda, Ruangveerayuth Ronnatrai, Pattanapanyasat Kovit, Pérignon Jean-Louis, Roberts David J, Druilhe Pierre
Bio-medical Parasitology Unit, Institut Pasteur, Paris, France.
PLoS Pathog. 2009 Oct;5(10):e1000631. doi: 10.1371/journal.ppat.1000631. Epub 2009 Oct 23.
Monocyte (MO) subpopulations display distinct phenotypes and functions which can drastically change during inflammatory states. We hypothesized that discrete MO subpopulations are induced during malaria infection and associated with anti-parasitic activity. We characterized the phenotype of blood MO from healthy malaria-exposed individuals and that of patients with acute uncomplicated malaria by flow cytometry. In addition, MO defense function was evaluated by an in vitro antibody dependent cellular inhibition (ADCI) assay. At the time of admission, the percentages and absolute numbers of CD16+ MO, and CCR2+CX3CR1+ MO, were high in a majority of patients. Remarkably, expression of CCR2 and CX3CR1 on the CD14(high (hi)) MO subset defined two subgroups of patients that also differed significantly in their functional ability to limit the parasite growth, through the ADCI mechanism. In the group of patients with the highest percentages and absolute numbers of CD14(hi)CCR2+CX3CR1+ MO and the highest mean levels of ADCI activity, blood parasitemias were lower (0.14+/-0.34%) than in the second group (1.30+/-3.34%; p = 0.0053). Data showed that, during a malaria attack, some patients' MO can exert a strong ADCI activity. These results bring new insight into the complex relationships between the phenotype and the functional activity of blood MO from patients and healthy malaria-exposed individuals and suggest discrete MO subpopulations are induced during malaria infection and are associated with anti-parasitic activity.
单核细胞（MO）亚群表现出不同的表型和功能，在炎症状态下会发生巨大变化。我们推测，在疟疾感染期间会诱导出离散的MO亚群，且这些亚群与抗寄生虫活性相关。我们通过流式细胞术对健康的疟疾暴露个体和急性非复杂性疟疾患者的血液MO表型进行了表征。此外，通过体外抗体依赖性细胞抑制（ADCI）试验评估了MO的防御功能。在入院时，大多数患者的CD16⁺ MO以及CCR2⁺CX3CR1⁺ MO的百分比和绝对数量都很高。值得注意的是，CD14高表达（hi）的MO亚群上CCR2和CX3CR1的表达定义了两组患者，这两组患者通过ADCI机制限制寄生虫生长的功能能力也存在显著差异。在CD14高表达（hi）CCR2⁺CX3CR1⁺ MO百分比和绝对数量最高且ADCI活性平均水平最高的患者组中，血液中的疟原虫血症（0.14±0.34%）低于第二组（1.30±3.34%；p = 0.0053）。数据表明，在疟疾发作期间，一些患者的MO可以发挥强大的ADCI活性。这些结果为患者和健康的疟疾暴露个体血液MO的表型与功能活性之间的复杂关系带来了新的见解，并表明在疟疾感染期间会诱导出离散的MO亚群，且这些亚群与抗寄生虫活性相关。
