法尼醇抑制大鼠部分肝切除术后的细胞增殖并诱导细胞凋亡。

Farnesol inhibits cell proliferation and induces apoptosis after partial hepatectomy in rats.

作者信息

Chagas Carlos Eduardo Andrade, Vieira Alessandra, Ong Thomas Prates, Moreno Fernando Salvador

机构信息

Faculty of Pharmaceutical Sciences, USP, Sao Paulo, Brazil.

出版信息

Acta Cir Bras. 2009 Sep-Oct;24(5):377-82. doi: 10.1590/s0102-86502009000500007.

DOI:10.1590/s0102-86502009000500007

PMID:19851690

Abstract

PURPOSE

To study farnesol (FOH) effects on liver regeneration after 70% partial hepatectomy (PH) in rats.

METHODS

Animals received FOH (25 mg/100 g body weight/day) or corn oil (CO, 0.25 mL/100 g body weight/day, controls). After a 2 week-treatment, all animals were subjected to PH and euthanized at different time points (0 h, 0.5 h, 4 h, 8 h, 18 h and 24 h) after surgery. Hepatic cell proliferation (PCNA positive nuclei) and apoptosis (fluorescence microscopy) were evaluated.

RESULTS

Compared to CO treatment, FOH treatment inhibited (p<0.05) cell proliferation at 24h (S phase of the cell cycle) after PH. This was preceded by an induction of apoptosis 0.5 h (p<0.05; G(0)/G(1) transition phase) after surgery.

CONCLUSION

The results of the present study suggest that apoptosis induction could be associated with the reduced number of cells at the S phase observed in FOH group. These novel in vivo data reinforce FOH as a promising chemopreventive and therapeutic agent against cancer.

摘要

目的

研究法尼醇（FOH）对大鼠70%肝部分切除术（PH）后肝再生的影响。

方法

动物接受FOH（25毫克/100克体重/天）或玉米油（CO，0.25毫升/100克体重/天，作为对照）。经过2周的治疗后，所有动物均接受肝部分切除术，并在术后不同时间点（0小时、0.5小时、4小时、8小时、18小时和24小时）安乐死。评估肝细胞增殖（PCNA阳性细胞核）和细胞凋亡（荧光显微镜检查）情况。