Chagas Carlos Eduardo Andrade, Vieira Alessandra, Ong Thomas Prates, Moreno Fernando Salvador
Faculty of Pharmaceutical Sciences, USP, Sao Paulo, Brazil.
Acta Cir Bras. 2009 Sep-Oct;24(5):377-82. doi: 10.1590/s0102-86502009000500007.
To study farnesol (FOH) effects on liver regeneration after 70% partial hepatectomy (PH) in rats.
Animals received FOH (25 mg/100 g body weight/day) or corn oil (CO, 0.25 mL/100 g body weight/day, controls). After a 2 week-treatment, all animals were subjected to PH and euthanized at different time points (0 h, 0.5 h, 4 h, 8 h, 18 h and 24 h) after surgery. Hepatic cell proliferation (PCNA positive nuclei) and apoptosis (fluorescence microscopy) were evaluated.
Compared to CO treatment, FOH treatment inhibited (p<0.05) cell proliferation at 24h (S phase of the cell cycle) after PH. This was preceded by an induction of apoptosis 0.5 h (p<0.05; G(0)/G(1) transition phase) after surgery.
The results of the present study suggest that apoptosis induction could be associated with the reduced number of cells at the S phase observed in FOH group. These novel in vivo data reinforce FOH as a promising chemopreventive and therapeutic agent against cancer.
研究法尼醇（FOH）对大鼠70%肝部分切除术（PH）后肝再生的影响。
动物接受FOH（25毫克/100克体重/天）或玉米油（CO，0.25毫升/100克体重/天，作为对照）。经过2周的治疗后，所有动物均接受肝部分切除术，并在术后不同时间点（0小时、0.5小时、4小时、8小时、18小时和24小时）安乐死。评估肝细胞增殖（PCNA阳性细胞核）和细胞凋亡（荧光显微镜检查）情况。
与CO治疗组相比，FOH治疗组在肝部分切除术后24小时（细胞周期的S期）抑制了（p<0.05）细胞增殖。这之前在术后0.5小时（p<0.05；G(0)/G(1)转换期）诱导了细胞凋亡。
本研究结果表明，细胞凋亡的诱导可能与FOH组中观察到的S期细胞数量减少有关。这些新的体内数据强化了FOH作为一种有前景的癌症化学预防和治疗药物的地位。
