Department EVA Environment and Agrobiotechnologies, Centre de Recherche Public-Gabriel Lippmann, 4422 Belvaux, Luxembourg.
Plant Cell Rep. 2009 Dec;28(12):1799-816. doi: 10.1007/s00299-009-0780-5. Epub 2009 Oct 23.
The molecular response to salt exposure was studied in the leaves of a Solanum tuberosum clone using cDNA microarray. Differentially expressed genes were classified according to their known or predicted function and their expression ratio as compared to the control. The major changes upon a 150 mM NaCl exposure in potato leaves occurred in the photosystem apparatus and Calvin cycle: many transcripts coding for proteins belonging to photosystems I and II and chlorophyll synthesis were repressed. On the other hand, we observed the induction of various kinds of transcription factors implicated in osmotic stress response via ABA-dependent or ABA-independent pathways but also in plant defense pathways. This revealed a crosstalk between abiotic and biotic stress responses during salt exposure, which activated several adaptation mechanisms including heat shock proteins, late embryogenesis abundant, dehydrins and PR proteins. Gene expression changes related to carbohydrate and amino acid metabolism were also observed, pointing at putative modifications at the metabolic level.
采用 cDNA 微阵列研究了盐胁迫下马铃薯克隆叶片的分子响应。根据已知或预测的功能以及与对照相比的表达比率，对差异表达基因进行分类。在马铃薯叶片中，150mM NaCl 暴露后的主要变化发生在光合作用系统和卡尔文循环中：许多编码属于光系统 I 和 II 以及叶绿素合成的蛋白质的转录本受到抑制。另一方面，我们观察到通过 ABA 依赖或非依赖途径以及植物防御途径参与渗透胁迫反应的各种转录因子的诱导。这揭示了盐胁迫过程中生物和非生物胁迫反应之间的串扰，激活了包括热激蛋白、晚期胚胎丰富蛋白、脱水蛋白和 PR 蛋白在内的几种适应机制。还观察到与碳水化合物和氨基酸代谢相关的基因表达变化，表明在代谢水平上可能发生了修饰。
