Department Environment and Agro-biotechnologies (EVA), Centre de Recherche Public-Gabriel Lippmann, 41, rue du Brill, L-4422 Belvaux, Luxembourg.
Int J Mol Sci. 2013 Mar 1;14(3):4912-33. doi: 10.3390/ijms14034912.
Better knowledge on responses to dehydration stress could help to improve the existing cryopreservation protocols for potato, since plant tissues processed for cryopreservation are often submitted to similar in vitro stress conditions. Cryopreservation (the best method of conservation for vegetatively propagated plants) of potato still needs to be standardized to make it available and to conserve the wide diversity of this crop. In the present work, the response to osmotic stress and chilling temperature was investigated in two potato species, Solanum tuberosum and its relative, frost-tolerant S. commersonii. After 14 days of exposure, different growth parameters, such as shoot length and number of leaves, were measured. Furthermore, differentially abundant proteins were identified after performing 2-fluorescence difference gel electrophoresis (2-DIGE) experiments, and soluble carbohydrates were analyzed by High Performance Anion Exchange Chromatography with Pulsed Amperometric Detection (HPAEC-PAD). The results show different responses in both species depending on the stress treatment. Focusing on the differences in growth parameters during the treatments, Solanum commersonii seems to be more affected than S. tuberosum cv. Désirée. At the molecular level, there are some differences and similarities between the two potato species studied that are dependent on the type of stressor.
更好地了解植物对脱水胁迫的响应可以帮助改进现有的马铃薯冷冻保存方案，因为用于冷冻保存的植物组织通常会经历类似的体外胁迫条件。马铃薯的冷冻保存（用于营养繁殖植物的最佳保存方法）仍需要标准化，以使其可用并保存这种作物的广泛多样性。在本工作中，研究了两种马铃薯，即栽培马铃薯及其亲缘种抗冻性的 S. commersonii 对渗透胁迫和冷藏温度的响应。经过 14 天的暴露，测量了不同的生长参数，如芽长和叶片数。此外，通过执行 2-荧光差异凝胶电泳（2-DIGE）实验鉴定了差异丰度蛋白，并通过高效阴离子交换色谱-脉冲安培检测（HPAEC-PAD）分析了可溶性碳水化合物。结果表明，两种物种根据不同的胁迫处理表现出不同的响应。根据处理过程中的生长参数差异，S. commersonii 似乎比 S. tuberosum cv. Désirée 受影响更大。在分子水平上，研究的两种马铃薯物种之间存在一些差异和相似之处，这取决于胁迫因子的类型。
