Faculty of Life Science, Northwestern Polytechnical University, Xi'an 710072, Shaanxi, China.
Nat Prod Res. 2009;23(17):1589-98. doi: 10.1080/14786410802496903.
Anthocyanin pigments in the fruit of mockstrawberry (Duchesnea indica Focke), were extracted with 0.1% HCl in ethanol, and the crude anthocyanin extract was purified by a C18 Sep-Pak cartridge open-column chromatography. High-performance liquid chromatography (HPLC) with photodiode array detection and mass spectrometry (MS) was applied for the isolation and composition analysis of anthocyanins in the fruit of mockstrawberry and their aglycones from acid hydrolysis. Three anthocyanins were found in the fruit of mockstrawberry and they were identified as cyanidin 3-O-rutinoside (61%), peonidin 3-O-rutinoside (34%), and petunidin 3-O-rutinoside (5%), respectively, by spectroscopic methods (UV-vis and MS). The two major anthocyanins were isolated by preparative HPLC, and their chemical structures were further characterised by Heuro(1) NMR. On the basis of chromatographic data, the total anthocyanin content was 205 mg g(-1) of the fresh fruit of mockstrawberry.
悬钩子属植物果实中的花色苷色素，用 0.1% HCl 在乙醇中提取，并用 C18 Sep-Pak 筒开柱色谱法对粗花色苷提取物进行纯化。高效液相色谱法（HPLC）与光电二极管阵列检测和质谱（MS）联用，用于从酸水解中分离和分析悬钩子属植物果实中的花色苷及其糖苷配基。从悬钩子属植物果实中发现了三种花色苷，分别为矢车菊素 3-O-芸香糖苷（61%）、芍药素 3-O-芸香糖苷（34%）和牵牛花素 3-O-芸香糖苷（5%），通过光谱法（UV-vis 和 MS）鉴定。两种主要的花色苷通过制备型 HPLC 分离，并通过 Heuro(1) NMR 进一步表征其化学结构。根据色谱数据，悬钩子属植物新鲜果实中的总花色苷含量为 205mg g(-1)。
