Hrynash Halyna, Pilly Vinay Kumar, Mankovskaia Alexandra, Xiong Yaoyang, Nogueira Filho Getulio, Bresciani Eduardo, Lévesque Céline Marie, Prakki Anuradha
Department of Clinical Sciences-Restorative, Faculty of Dentistry, University of Toronto, 124 Edward Street, Room 352C, Toronto, ON, Canada M5G 1G6.
Department of Biological and Diagnostic Sciences-Dental Public Health, Faculty of Dentistry, University of Toronto, 124 Edward Street, Toronto, ON, Canada M5G 1G6.
Int J Dent. 2014;2014:289401. doi: 10.1155/2014/289401. Epub 2014 Feb 18.
Objective. To evaluate bacterial growth inhibition, mechanical properties, and compound release rate and stability of copolymers incorporated with anthocyanin (ACY; Vaccinium macrocarpon). Methods. Resin samples were prepared (Bis-GMA/TEGDMA at 70/30 mol%) and incorporated with 2 w/w% of either ACY or chlorhexidine (CHX), except for the control group. Samples were individually immersed in a bacterial culture (Streptococcus mutans) for 24 h. Cell viability (n = 3) was assessed by counting the number of colony forming units on replica agar plates. Flexural strength (FS) and elastic modulus (E) were tested on a universal testing machine (n = 8). Compound release and chemical stability were evaluated by UV spectrophotometry and (1)H NMR (n = 3). Data were analyzed by one-way ANOVA and Tukey's test ( α = 0.05). Results. Both compounds inhibited S. mutans growth, with CHX being most effective (P < 0.05). Control resin had the lowest FS and E values, followed by ACY and CHX, with statistical difference between control and CHX groups for both mechanical properties (P < 0.05). The 24 h compound release rates were ACY: 1.33 μg/mL and CHX: 1.92 μg/mL. (1)H NMR spectra suggests that both compounds remained stable after being released in water. Conclusion. The present findings indicate that anthocyanins might be used as a natural antibacterial agent in resin based materials.
目的。评估与花青素（ACY；大果越桔）结合的共聚物的细菌生长抑制、机械性能、化合物释放速率和稳定性。方法。制备树脂样品（双酚A甘油醚/三乙二醇二甲基丙烯酸酯，摩尔比为70/30），除对照组外，分别加入2%（质量/质量）的ACY或氯己定（CHX）。将样品分别浸入细菌培养物（变形链球菌）中24小时。通过计算复制琼脂平板上的菌落形成单位数量来评估细胞活力（n = 3）。在万能试验机上测试弯曲强度（FS）和弹性模量（E）（n = 8）。通过紫外分光光度法和核磁共振氢谱（1H NMR）评估化合物释放和化学稳定性（n = 3）。数据通过单因素方差分析和Tukey检验进行分析（α = 0.05）。结果。两种化合物均抑制变形链球菌生长，其中CHX最有效（P < 0.05）。对照树脂的FS和E值最低，其次是ACY和CHX，两种机械性能在对照组和CHX组之间均有统计学差异（P < 0.05）。24小时化合物释放速率分别为：ACY：1.33μg/mL，CHX：1.92μg/mL。核磁共振氢谱表明两种化合物在水中释放后均保持稳定。结论。本研究结果表明花青素可能用作树脂基材料中的天然抗菌剂。
