Huang Jin, Liu Zhen-Shi, Yao Lan, Dong Su-Juan, He Qi-Lin, Xiong Tao, Wei Lan-Ying, Eang Zheng-Ming, Jiang Chang-Fu
Department of Parasitology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2009 Jun;27(3):223-6.
To establish a magnetic particle antibody immunoassay (MPAIA) for the detection of specific antibody in sera of schistosomiasis patients.
Fluorescein isothiocyanate (FITC) was used to label Schistosoma japonicum soluble egg antigen (Sj-SEA). Anti-human IgG coated with alkaline phosphatase (ALP) as enzyme-labeled second antibody, and magnetic beads were coupled with sheep anti-FITC antibody as solid phase. Phenolphthale in monophosphate was used as substrate to set up MPAIA for the detection. Serum samples from cases with schistosomiasis or other helminth infections were tested.
The positive rate of MPAIA was 96.7% (116/120) with the sera of S. japonicum-infected cases. No cross reaction was observed with sera of trichinellosis, paragonimiasis or cysticercosis cases. The positive titer with reference sample was 1: 1,600. The precision was lower than 10%. The MPAIA tips can be stored at 4 degrees C for 12 months.
MPAIA shows a high sensitivity, proper specificity and long-term validity for schistosomiasis detection.
建立一种用于检测血吸虫病患者血清中特异性抗体的磁微粒抗体免疫测定法（MPAIA）。
用异硫氰酸荧光素（FITC）标记日本血吸虫可溶性虫卵抗原（Sj-SEA）。以碱性磷酸酶（ALP）包被的抗人IgG作为酶标二抗，磁珠与羊抗FITC抗体偶联作为固相。以磷酸一酚酞为底物建立MPAIA用于检测。检测血吸虫病患者及其他蠕虫感染患者的血清样本。
MPAIA对日本血吸虫感染患者血清的阳性检出率为96.7%（116/120）。旋毛虫病、肺吸虫病或囊尾蚴病患者血清未出现交叉反应。参考样本的阳性效价为1:1600。精密度低于10%。MPAIA检测条可在4℃保存12个月。
MPAIA对血吸虫病检测具有高灵敏度、适当的特异性和长期有效性。
