Cai Yu-Chun, Guo Jian, Chen Shao-Hong, Tian Li-Guang, Steinmann Peter, Chen Mu-Xin, Li Hao, Ai Lin, Chen Jia-Xu
National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025, PR China.
Parasitol Int. 2012 Sep;61(3):385-90. doi: 10.1016/j.parint.2012.01.008. Epub 2012 Feb 9.
IgY isolated from egg yolk has been widely used in immunodiagnostic tests, including tests to detect circulating antigen (soluble egg antigen or SEA) of Schistosoma japonicum.
A sandwich ELISA was established using a combination of anti-S. japonicum SEA-IgY polyclonal antibodies and IgM monoclonal antibodies. To explore sensitivity and specificity of the sandwich ELISA, serum samples from 43 patients infected with S. japonicum were tested. All acute cases and 91.3% of the chronic cases showed a positive reaction. Only 5% of the control sera from healthy persons gave a positive response. Cross-reactions with antibodies to nine other parasites were rare.
The developed immunoassay is reasonably sensitive and specific. It could be used for field research and treatment efficacy assessments.
从蛋黄中分离出的免疫球蛋白Y（IgY）已广泛应用于免疫诊断测试，包括检测日本血吸虫循环抗原（可溶性虫卵抗原或SEA）的测试。
使用抗日本血吸虫SEA-IgY多克隆抗体和IgM单克隆抗体组合建立了夹心ELISA。为了探索夹心ELISA的敏感性和特异性，对43例日本血吸虫感染患者的血清样本进行了检测。所有急性病例和91.3%的慢性病例均呈阳性反应。健康人的对照血清中只有5%呈阳性反应。与其他九种寄生虫抗体的交叉反应很少。
所开发的免疫测定法具有合理的敏感性和特异性。它可用于现场研究和治疗效果评估。
