在津巴布韦一个农村社区中，吡喹酮治疗对埃及血吸虫和HIV-1合并感染个体MIP-1α/CCL3的下调作用

Downregulation of MIP-1alpha/CCL3 with praziquantel treatment in Schistosoma haematobium and HIV-1 co-infected individuals in a rural community in Zimbabwe.

作者信息

Zinyama-Gutsire Rbl, Gomo E, Kallestrup P, Erikstrup C, Ullum H, Butterworth A E, Munyati S, Mduluza T

机构信息

Biochemistry Department, University of Zimbabwe, Harare, Zimbabwe.

出版信息

BMC Infect Dis. 2009 Oct 23;9:174. doi: 10.1186/1471-2334-9-174.

DOI:10.1186/1471-2334-9-174

PMID:19852800

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2770052/

Abstract

BACKGROUND

Chemokines have been reported to play an important role in granulomatous inflammation during Schistosoma mansoni infection. However there is less information on their role in Schistosoma haematobium infection, or on the effect of concurrent HIV-1 infection, as a potential modifying influence.

METHODS

To determine levels of MIP-1alpha/CCL3 chemokine in plasma of S. haematobium and HIV-1 co-infected and uninfected individuals in a rural black Zimbabwean community.A cohort was established of HIV-1 and schistosomiasis infection and co-infection comprising 379 participants. Outcome measures consisted of HIV-1 and schistosomiasis status and levels of MIP-1alpha/CCL3 in plasma at baseline and three months post treatment. An association was established between MIP-1alpha/CCL3 plasma levels with HIV-1 and S. haematobium infections.

RESULTS

A total of 379 adults formed the established cohort comprising 76 (20%) men and 303 (80%) women. Mean age was 33.25, range 17 - 62 years. The median MIP-1alpha/CCL3 plasma concentration was significantly higher in S. haematobium infected compared with uninfected individuals (p = 0.029). In contrast, there was no difference in the median MIP-1alpha/CCL3 levels between HIV-1 positive and negative individuals (p = 0.631). MIP-1alpha/CCL3 concentration in plasma was significantly reduced at three months after treatment with praziquantel (p = 000).

CONCLUSION

The results of our study show that the MIP-1alpha/CCL3 levels were positively associated with S. haematobium egg counts at baseline but not with HIV-1 infection status. MIP-1alpha/CCL3 levels were significantly reduced at three months post treatment with praziquantel. We therefore conclude that MIP-1alpha/CCL3 is produced during infection with S haematobium. S. haematobium infection is associated with increased MIP-1alpha/CCL3 levels in an egg intensity-dependent manner and treatment of S. haematobium is associated with a reduction in MIP-1alpha/CCL3.

摘要

背景

据报道，趋化因子在曼氏血吸虫感染期间的肉芽肿性炎症中起重要作用。然而，关于它们在埃及血吸虫感染中的作用，或作为一种潜在的修饰影响，关于同时感染HIV-1的影响的信息较少。

方法

为了确定津巴布韦农村黑人社区中感染埃及血吸虫和HIV-1以及未感染的个体血浆中MIP-1α/CCL3趋化因子的水平。建立了一个由379名参与者组成的HIV-1和血吸虫病感染及合并感染队列。观察指标包括HIV-1和血吸虫病状态以及基线和治疗后三个月血浆中MIP-1α/CCL3的水平。确定了血浆中MIP-1α/CCL3水平与HIV-1和埃及血吸虫感染之间的关联。

结果

共有379名成年人组成了该队列，其中包括76名（20%）男性和303名（80%）女性。平均年龄为33.25岁，范围为17至62岁。与未感染个体相比，感染埃及血吸虫的个体血浆中MIP-1α/CCL3的中位数浓度显著更高（p = 0.029）。相比之下，HIV-1阳性和阴性个体之间MIP-1α/CCL3的中位数水平没有差异（p = 0.631）。用吡喹酮治疗三个月后，血浆中MIP-1α/CCL3的浓度显著降低（p = 0.000）。

结论

我们的研究结果表明，MIP-1α/CCL3水平在基线时与埃及血吸虫卵计数呈正相关，但与HIV-1感染状态无关。用吡喹酮治疗三个月后，MIP-1α/CCL3水平显著降低。因此，我们得出结论，MIP-1α/CCL3是在感染埃及血吸虫期间产生的。埃及血吸虫感染与MIP-1α/CCL3水平以卵强度依赖的方式增加有关，而治疗埃及血吸虫与MIP-1α/CCL3的降低有关。