Forte W C N, Guardian V C, Mantovani P A, Dionigi P C L, Menezes M C S
Santa Casa Medical School and Hospital, São Paulo, Brazil.
Allergol Immunopathol (Madr). 2009 Nov-Dec;37(6):302-8. doi: 10.1016/j.aller.2009.06.003.
Patients with atopic dermatitis frequently present recurrent infections by pyogenic bacteria or by intracellular microorganisms, suggesting an immune disorder.
Laboratorial investigation of phagocyte activity and chemotactic response by neutrophilic polymorphonuclear and mononuclear phagocytes in the peripheral blood of patients with atopic dermatitis from moderate to severe.
Through a transversal study, patients with atopic dermatitis from moderate to severe were selected. The neutrophilic and mononuclear phagocytes were separated and the phagocytic ingestion of zymosan particles was analysed, in addition to migration distance to the bacterial lipopolysaccharide chemotactic factor, comparing the results to the values obtained from healthy individuals within the same age group.
Nineteen patients were selected, 11 female and 8 male. The mean age was 6.47 years (+/-4.65). Among the 19 patients studied, 14 (73.68%) presented a reduction in the neutrophilic and mononuclear phagocyte activity, with two (1.53%) patients presenting a reduction in the activity of both phagocytes.
Our results demonstrated a reduction in chemotactic response and phagocytic activity by neutrophilic and/or mononuclear phagocytes in the majority of patients with atopic dermatitis from moderate to severe. Our results were coherent with the clinical data concerning the higher incidence of infections by pyogenic bacteria and fungi in patients with atopic dermatitis, which are microorganisms that require defence by the phagocytes researched in the present study.
特应性皮炎患者经常出现由化脓性细菌或细胞内微生物引起的反复感染，提示存在免疫紊乱。
对中度至重度特应性皮炎患者外周血中的嗜中性多形核和单核吞噬细胞的吞噬活性和趋化反应进行实验室研究。
通过一项横向研究，选取中度至重度特应性皮炎患者。分离嗜中性和单核吞噬细胞，分析酵母聚糖颗粒的吞噬摄取情况，以及对细菌脂多糖趋化因子的迁移距离，并将结果与同一年龄组健康个体获得的值进行比较。
选取了19名患者，其中11名女性，8名男性。平均年龄为6.47岁（±4.65）。在研究的19名患者中，14名（73.68%）出现嗜中性和单核吞噬细胞活性降低，2名（1.53%）患者两种吞噬细胞活性均降低。
我们的结果表明，大多数中度至重度特应性皮炎患者的嗜中性和/或单核吞噬细胞的趋化反应和吞噬活性降低。我们的结果与特应性皮炎患者中化脓性细菌和真菌感染发生率较高的临床数据一致，这些微生物需要本研究中所研究的吞噬细胞进行防御。
