Department of Dermatology, Ehime University Graduate School of Medicine, Toon City, Ehime, Japan.
J Dermatol Sci. 2009 Dec;56(3):188-95. doi: 10.1016/j.jdermsci.2009.09.009. Epub 2009 Oct 23.
Living skin equivalents (LSEs) are being used to treat burn wounds, skin defects, and chronic wounds, and today, several biomaterials are applied as dermal matrices in LSEs. The amnionic membrane (AM) is known to have useful properties as a dermal matrix and can be used to construct a LSE.
To develop a new LSE with human AM as the matrix.
Human AM was de-epithelialized and investigated to determine whether it supported keratinocyte adherence and proliferation, and fibroblast in-growth and proliferation. A new LSE was constructed by seeding keratinocytes on the epithelial side of fibroblast-populated, de-epithelialized AM and was investigated histologically. The LSE was transplanted onto a full-thickness wound on a nude mouse and a histological examination was conducted.
De-epithelialized AM supported the adherence and proliferation of keratinocytes and the in-growth of fibroblasts. The new LSE demonstrated good mechanical properties and revealed good morphology, with a well differentiated epidermis and well developed basement membrane. The LSE grafts survived well on nude mice, showing good morphology.
A LSE with amnions as a matrix exhibited good morphology, low cost, and good mechanical properties and may be useful as a skin substitute for clinical use.
活体皮肤等效物（LSE）正被用于治疗烧伤创面、皮肤缺损和慢性创面，目前，几种生物材料被用作 LSE 中的真皮基质。羊膜（AM）作为真皮基质具有有用的特性，可用于构建 LSE。
开发一种以人 AM 为基质的新型 LSE。
对人 AM 进行去上皮化处理，并对其是否支持角质形成细胞黏附和增殖、成纤维细胞向内生长和增殖进行研究。通过将角质形成细胞接种到成纤维细胞填充的去上皮化 AM 的上皮侧，构建一种新型 LSE，并进行组织学研究。将 LSE 移植到裸鼠的全层创面，并进行组织学检查。
去上皮化的 AM 支持角质形成细胞的黏附和增殖，以及成纤维细胞的向内生长。新型 LSE 具有良好的机械性能，形态良好，表皮分化良好，基膜发育良好。LSE 移植物在裸鼠上存活良好，形态良好。
以羊膜为基质的 LSE 具有良好的形态、低成本和良好的机械性能，可作为临床应用的皮肤替代品。
